QQ客服 官方微博 反馈留言

	Effects of glycerol on the compaction and stability of the wild type and mutated rabbit muscle creatine kinase
	Feng, Shan ; Yan, Yong-Bin
	2010-05-11 ; 2010-05-11
关键词	chemical chaperone conformational disease muscle creatine kinase protein misfolding and aggregation transition free energy of inactivation CARBONIC-ANHYDRASE-B UNFOLDED PROTEIN RESPONSE AGGREGATION DISEASES STRESS NEURODEGENERATION INTERMEDIATE DENATURATION OSMOLYTES MECHANISM Biochemistry & Molecular Biology Biophysics
中文摘要	All organisms have developed detect, repair, regulation, and stabilization mechanisms to survive from cellular and molecular damage induced by diverse stresses. Among them, the accumulation of osmolytes is a common mechanism evolved by cells to maintain cell volume and stabilize macromolecules against various environmental stresses. The molecular mechanisms by which osmolytes stabilize proteins and prevent aggregation have been well-established. However, little is known about the effects of osmolytes on mutated or damaged proteins. In this research, we investigated the effects of glycerol on the activity, structure, and stability of the wild type (WT) and D54G CK under normal and extreme (high temperature) conditions. It was found that glycerol had similar effects on the suppression of the aggregation during the refolding of both proteins. Under native conditions, the effect of glycerol on the mutated protein was more obvious than on the WT protein. Glycerol could efficiently force the mutated protein to fold to a state close to the WT protein, and thus stabilize the native state of the mutated protein. Glycerol could also protect both the WT and mutated proteins against heat-induced denaturation. However, the change in the transition free energy of heat-induced inactivation of the WT protein was larger than that of the mutated protein. These results suggested that glycerol might have differential effects on the changes of the chemical potential and the transition free energy of the WT and mutated proteins.
语种	英语 ; 英语
出版者	WILEY-LISS ; HOBOKEN ; DIV JOHN WILEY & SONS INC, 111 RIVER ST, HOBOKEN, NJ 07030 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26761]
专题	清华大学
推荐引用方式 GB/T 7714	Feng, Shan,Yan, Yong-Bin. Effects of glycerol on the compaction and stability of the wild type and mutated rabbit muscle creatine kinase[J],2010, 2010.
APA	Feng, Shan,&Yan, Yong-Bin.(2010).Effects of glycerol on the compaction and stability of the wild type and mutated rabbit muscle creatine kinase..
MLA	Feng, Shan,et al."Effects of glycerol on the compaction and stability of the wild type and mutated rabbit muscle creatine kinase".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。