QQ客服 官方微博 反馈留言

	Interaction between La3+ and MP-11 in the physiological solution
	Guo, Shaofen ; Zhou, Qing ; Lu, Tianhong ; Ding, Xiaolan ; Huang, Xiaohua
	2010-05-11 ; 2010-05-11
关键词	lanthanum microperoxidase-11 interaction electrochemical reaction HEART CYTOCHROME-C DIRECT ELECTROCHEMISTRY HORSERADISH-PEROXIDASE ELECTROCATALYTIC REDUCTION ELECTRON-TRANSFER GOLD ELECTRODE NICKEL(II) PORPHYRINS NONPLANAR DISTORTION GRAPHITE-ELECTRODES MOLECULAR-DYNAMICS Electrochemistry
中文摘要	In this paper, the interaction between La3+ and microperoxidase-11 (MP-11) in the imitated physiological solution was investigated with the electrochemical method, circular dichroism (CD) and ultraviolet-visible (UV-vis) absorption spectroscopy. It was found that the interaction ways between La3+ and MP-11 are different with increasing the molar ratio of La3+ and MP-11. When the molar ratio of La3+ and MP-11 is less than 2, La3+ mainly interacts with the metacetonic acid group of the heme group in the MP-11 molecules, causing the increase in the non-planarity of the porphyrin cycle in the heme group and the decrease in the content of the random coil conformation of MP-11. These structural changes would increase the exposure extent of the electrochemical active center of MP-11 and thus, La3+ can promote the electrochemical reaction of MP-11 and its electrocatalytic activity for the reduction of H2O2 at the glassy carbon (GC) electrode. However, when the molar ratio of La3+ and MP-11 is larger than 3, except binding to the carbonyl oxygen of the metacetonic acid group in the heme group, La3+ interacts also with the oxygen-containing groups of the amides in the polypeptide chains of the MP-11 molecules, leading to the increase in the contents of the random coil conformation in the peptide of the MP-11 molecule, comparing with that for the molar ratio of less than 2. However, the non-planarity of the porphyrin cycle in the heme group is similar to that for the molar ratio of less than 2. Perhaps, the increase in the contents of the random coil conformation in the peptide of the MP-11 molecule would partially shield the electrochemical active center of MP-11 and is not favorable to the electrochemical and electrocatalytic reaction of MP-11. Thus, the reversibility, of the electrochemical reaction of MP-11 and its electrocatalytic activity for the reduction of H2O2 are lower than that for the molar ratio of less than 2. (c) 2006 Elsevier Ltd. All rights reserved.
语种	英语 ; 英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD ; OXFORD ; THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26755]
专题	清华大学
推荐引用方式 GB/T 7714	Guo, Shaofen,Zhou, Qing,Lu, Tianhong,et al. Interaction between La3+ and MP-11 in the physiological solution[J],2010, 2010.
APA	Guo, Shaofen,Zhou, Qing,Lu, Tianhong,Ding, Xiaolan,&Huang, Xiaohua.(2010).Interaction between La3+ and MP-11 in the physiological solution..
MLA	Guo, Shaofen,et al."Interaction between La3+ and MP-11 in the physiological solution".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。