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	Capture of monomeric refolding intermediate of human muscle creatine kinase
	Li, S ; Bai, JH ; Park, YD ; Zhou, HM
	2010-05-11 ; 2010-05-11
关键词	human muscle creatine kinase monomeric refolding intermediate aggregation GroEL alpha(s)-casein CONFORMATIONAL-CHANGES PROTEIN DENATURATION GROEL CASEIN DEHYDROGENASE INACTIVATION FLUORESCENCE AGGREGATION PROTEOLYSIS Biochemistry & Molecular Biology
中文摘要	Human muscle creatine kinase (CK) is an enzyme that plays an important physiological role in the energy metabolism of humans. It also serves as a typical model for studying refolding of proteins. A study of the refolding and reactivation process of guanidine chloride-denatured human muscle CK is described in the present article. The results show that the refolding process can be divided into fast and slow folding phases and that an aggregation process competes with the proper refolding process at high enzyme concentration and high temperature. An intermediate in the early stage of refolding was captured by specific protein molecules: the molecular chaperonin GroEL and alpha(s)-casein. This intermediate was found to be a monomer, which resembles the "molten globule" state in the CK folding pathway. To our knowledge, this is the first monomeric intermediate captured during refolding of CK. We propose that aggregation is caused by interaction between such monomeric intermediates. Binding of GroEL with this intermediate prevents formation of aggregates by decreasing the concentration of free monomeric intermediates, whereas binding of alpha(s)-casein with this intermediate induces more aggregation.
语种	英语 ; 英语
出版者	COLD SPRING HARBOR LAB PRESS, PUBLICATIONS DEPT ; WOODBURY ; 500 SUNNYSIDE BLVD, WOODBURY, NY 11797-2924 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26708]
专题	清华大学
推荐引用方式 GB/T 7714	Li, S,Bai, JH,Park, YD,et al. Capture of monomeric refolding intermediate of human muscle creatine kinase[J],2010, 2010.
APA	Li, S,Bai, JH,Park, YD,&Zhou, HM.(2010).Capture of monomeric refolding intermediate of human muscle creatine kinase..
MLA	Li, S,et al."Capture of monomeric refolding intermediate of human muscle creatine kinase".(2010).

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