QQ客服 官方微博 反馈留言

	Identification of Nodal-regulated genes in zebrafish embryos
	Sun Zhi-Hui ; Meng An-Ming
	2010-05-11 ; 2010-05-11
关键词	zebrafish embryos Nodal genechip transcription factor ONE-EYED-PINHEAD ENDODERM FORMATION ORGANIZER PROTEIN MESENDODERM FGF SPECIFICATION INDUCTION ENCODES SIGNALS Biochemistry & Molecular Biology Biophysics
中文摘要	Nodal signals play important roles in mesendoderm induction, establishment of left-right asymmetry and anteroposterior patterning of the neuroectoderm during vertebrate embryogenesis. It is aimed at identifying Nodal-regulated genes in zebrafish embryos, particularly those encoding transcription factors. A (Affymetrix) genechip analysis was performed using RNAs derived from embryos injected with squint mRNA, MZoep mutant embryos that are deficient in Nodal signaling, and wildtype embryos at the 30% epiboly stage. Transcripts (genes) with at least two-fold changes in expression level between wildtype and the other samples were identified. In squint mRNA-injected embryos, 265 transcripts show an increased expression level and I I I have a decreased expression level; in MZoep embryos, the expression of 1 495 transcripts increases while 550 transcripts express at a decreased level. Furthermore, overexpression of squint causes increased expression of 26 and decreased expression of I I annotated transcription factor genes; in MZoep embryos, the number of transcription factor genes showing an increase and decrease of expression are 69 and 30, respectively. These results would provide useful information for further studying mechanisms and biological functions of Nodal signal transduction.
语种	中文 ; 中文
出版者	CHINESE ACADEMY SCIENCES, INST BIOPHYSICS ; BEIJING ; 15 DATUN RD, CHAOYAND DISTRICT, BEIJING, 100101, PEOPLES R CHINA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26673]
专题	清华大学
推荐引用方式 GB/T 7714	Sun Zhi-Hui,Meng An-Ming. Identification of Nodal-regulated genes in zebrafish embryos[J],2010, 2010.
APA	Sun Zhi-Hui,&Meng An-Ming.(2010).Identification of Nodal-regulated genes in zebrafish embryos..
MLA	Sun Zhi-Hui,et al."Identification of Nodal-regulated genes in zebrafish embryos".(2010).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。