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	The role of detergent in refolding of GdnHCl-denatured arginine kinase from shrimp Fenneropenaeus Chinensis: the solubilization of aggregate and refolding in detergent solutions
	Pan, JC ; Wang, JS ; Cheng, Y ; Yu, Z ; Rao, XM ; Zhou, HM
	2010-05-11 ; 2010-05-11
关键词	arginine kinase guanidine-denatured refolding detergent aggregation INCLUSION-BODY PROTEINS CREATINE-KINASE GUANIDINIUM CHLORIDE FENEROPENAEUS-CHINENSIS LAURYL MALTOSIDE FOLDING AIDS RHODANESE PATHWAY CHAPERONE PURIFICATION Biochemistry & Molecular Biology Cell Biology
中文摘要	Strong aggregation occurred in the refolding route of arginine kinase (AK) denatured with 3 mol GdnHCl/L (GdnHCl, guanidine hydrochloride). The activity recovery of GdnHCl-denatured AK was very low and dependent on the protein concentration in the process of refolding. For denatured AK at 1.2 mu mol/L concentration, the recovered activity yield was about 45.2% of the native enzyme, whereas at 5.2 mu mol/L the activity recovery yield was only 20% of native activity. The nonionic detergent Triton X-100 and Tween 20 (<= 100 mmol/L concentration) not only effectively blocked the aggregation but also enabled the denatured AK to recover most of its native activity. The kinetics of aggregate solubilization showed that there was an induction phase dependent on the detergent, but there was no dependency when detergent was absent. The apparent activity recovery had a cooperative relation with detergents in the process of refolding, which suggested the existence of some interaction between the detergent and the refolding intermediate. On the basis of the study results, a scheme of refolding was proposed.
语种	英语 ; 英语
出版者	NATL RESEARCH COUNCIL CANADA ; OTTAWA ; RESEARCH JOURNALS, MONTREAL RD, OTTAWA, ONTARIO K1A 0R6, CANADA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26644]
专题	清华大学
推荐引用方式 GB/T 7714	Pan, JC,Wang, JS,Cheng, Y,et al. The role of detergent in refolding of GdnHCl-denatured arginine kinase from shrimp Fenneropenaeus Chinensis: the solubilization of aggregate and refolding in detergent solutions[J],2010, 2010.
APA	Pan, JC,Wang, JS,Cheng, Y,Yu, Z,Rao, XM,&Zhou, HM.(2010).The role of detergent in refolding of GdnHCl-denatured arginine kinase from shrimp Fenneropenaeus Chinensis: the solubilization of aggregate and refolding in detergent solutions..
MLA	Pan, JC,et al."The role of detergent in refolding of GdnHCl-denatured arginine kinase from shrimp Fenneropenaeus Chinensis: the solubilization of aggregate and refolding in detergent solutions".(2010).

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