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	Effects of hydroxypropyl cyclodextrins on the reactivation of SDS-denatured aminoacylase
	Kim, Sung-Hye ; Zhou, Hai-Meng ; Yan, Yong-Bin
	2010-05-11 ; 2010-05-11
关键词	aminoacylase hydroxypropyl cyclodextrins sodium dodecyl sulfate BOVINE CARBONIC-ANHYDRASE SODIUM DODECYL-SULFATE ARTIFICIAL CHAPERONE INACTIVATION AGGREGATION DETERGENT PROTEINS AMYLASE SYSTEM ENZYME Biochemistry & Molecular Biology
中文摘要	Cyclodextrins are natural-occurring circular oligosaccharides with an internal hydrophobic cavity and external hydrophilic edges. Because cyclodextrins bind with protein aromatic residues, they can prevent protein aggregation, and their ability to bind with detergents enables them to act as stripping reagents to release proteins from protein-detergent complexes. In this research, we investigated the effects of three hydroxypropyl cyclodextrins (HPCDs) on the refolding of aminoacylase from SDS-denatured states. It was found that the three HPCDs could effectively assist aminoacylase reactivation though they have different abilities. HP-gamma-CD, which has the largest cavity among the three HPCDs, was the most efficient one. Spectroscopic results further indicated that the secondary structure recovery of aminoacylase could be completed with the help of low concentrations of HPCDs. However, the activity of the released protein could not fully recover even though high concentrations of HPCDs were used. The concentration-dependent effects of HPCDs also indicated that cyclodextrins could also act as folding assistants in addition to acting as stripping reagents during the refolding of detergent-denatured proteins. (c) 2006 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26604]
专题	清华大学
推荐引用方式 GB/T 7714	Kim, Sung-Hye,Zhou, Hai-Meng,Yan, Yong-Bin. Effects of hydroxypropyl cyclodextrins on the reactivation of SDS-denatured aminoacylase[J],2010, 2010.
APA	Kim, Sung-Hye,Zhou, Hai-Meng,&Yan, Yong-Bin.(2010).Effects of hydroxypropyl cyclodextrins on the reactivation of SDS-denatured aminoacylase..
MLA	Kim, Sung-Hye,et al."Effects of hydroxypropyl cyclodextrins on the reactivation of SDS-denatured aminoacylase".(2010).

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