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	Culture of outer epithelial cells from mantle tissue to study shell matrix protein secretion for biomineralization
	Gong, Ningping ; Li, Qi ; Huang, Jing ; Fang, Zi ; Zhang, Guiyou ; Xie, Liping ; Zhang, Rongqing
	2010-05-11 ; 2010-05-11
关键词	mantle cell culture matrix protein quantification biomineralization pearl oyster, Pinctada fucata (Mollusca) OYSTER PINCTADA-FUCATA HALIOTIS-TUBERCULATA CARBONIC-ANHYDRASE ORGANIC MATRIX MOLLUSK BIVALVE HEMOCYTES ABALONE NACREIN MUSSEL Cell Biology
中文摘要	Mantle tissue plays an important role in shell biomineralization by secreting matrix proteins for shell formation. However, the mechanism by which it regulates matrix protein secretion is poorly understood, largely because of the lack of cellular tools for in vitro study and techniques to evaluate matrix protein secretion. We have isolated the outer epithelial cells of the mantle of the pearl oyster, Pinctada fucata, and evaluated cellular metabolism by measuring the secretion of the matrix protein, nacrein. A novel sensitive sandwich enzyme-linked immunosorbent assay (ELISA) was established to quantify nacrein. Mantle explant culture was demonstrated to provide dissociated tissue cells with high viability. Single dissociated cell types from explant culture were separated by density in a discontinuous Percoll gradient. The outer epithelial cells were isolated from other cell types by their higher density and identified by immunolabeling and ultrastructure analysis. ELISA assays revealed that the outer epithelial cells retained the ability to secrete nacrein in vitro. Moreover, increased nacrein secretion resulted from an increased Ca2+ concentration in the culture media of the outer epithelial cells, in a concentration-dependent manner. These results confirm that outer epithelial cell culture and the ELISA method are useful tools for studying the regulatory mechanisms of shell biomineralization.
语种	英语 ; 英语
出版者	SPRINGER ; NEW YORK ; 233 SPRING ST, NEW YORK, NY 10013 USA
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/26580]
专题	清华大学
推荐引用方式 GB/T 7714	Gong, Ningping,Li, Qi,Huang, Jing,et al. Culture of outer epithelial cells from mantle tissue to study shell matrix protein secretion for biomineralization[J],2010, 2010.
APA	Gong, Ningping.,Li, Qi.,Huang, Jing.,Fang, Zi.,Zhang, Guiyou.,...&Zhang, Rongqing.(2010).Culture of outer epithelial cells from mantle tissue to study shell matrix protein secretion for biomineralization..
MLA	Gong, Ningping,et al."Culture of outer epithelial cells from mantle tissue to study shell matrix protein secretion for biomineralization".(2010).

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