| Chemical modification of insulin by N-phosphorylation | |
| Liu, Y ; Xu, PX ; Hou, JB ; Yang, LF ; Chen, JA ; Zhao, YF | |
| 2010-05-06 ; 2010-05-06 | |
| 关键词 | bovine insulin CD ESI-MS HPLC MALDI-TOF phosphorylation DERIVATIVES PEPTIDES PRODRUGS Biochemistry & Molecular Biology |
| 中文摘要 | In this paper, the reactions of bovine insulin and small peptides, such as actin binding domain of thymosin (34 and Growth Hormone Releasing Factor (GRF 1-29 amino acids) with diisopropyloxyphosphite (DIPPH) and dimethyloxyphosphite (DMPH) were studied by modified Todd reaction. The MALDI-TOF or ESI-MS results showed that lysine, histidine and arginine residues in insulin could be phosphorylated under the water/ethanol system. The N,N,N-diisopropyloxyphosphorylated insulin analogues were characterized using MALDI-TOF and P-31 NMR. These insulin analogues with different phosphorylation degree were separated and identified through LC-ESI-MS. In addition, circular dichroism (CD) spectra showed that the conformation of N,N,N-dimethyloxyphosphorylated insulin were only changed a little, whereas, that of N,N,N-diisopropyloxyphosphorylated insulin was changed completely. |
| 语种 | 英语 ; 英语 |
| 出版者 | SPRINGER ; NEW YORK ; 233 SPRING STREET, NEW YORK, NY 10013 USA |
| 内容类型 | 期刊论文 |
| 源URL | [http://hdl.handle.net/123456789/13513]  |
| 专题 | 清华大学 |
| 推荐引用方式 GB/T 7714 | Liu, Y,Xu, PX,Hou, JB,et al. Chemical modification of insulin by N-phosphorylation[J],2010, 2010. |
| APA | Liu, Y,Xu, PX,Hou, JB,Yang, LF,Chen, JA,&Zhao, YF.(2010).Chemical modification of insulin by N-phosphorylation.. |
| MLA | Liu, Y,et al."Chemical modification of insulin by N-phosphorylation".(2010). |
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