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	The enhancement effect of CaCl2 on solid substrate room temperature phosphorescence of polyhalogenated derivatives of fluorescein and application in immunoassay
	Wu, YL ; Li, LD ; Liu, JM ; Zhu, GH
	2010-05-06 ; 2010-05-06
关键词	solid substrate room temperature phosphorescence immunoassay polyhalogenated derivatives of fluorescein eosin-ITC CaCl2 as an enhancer human complement 3 Chemistry, Analytical
中文摘要	It was found that the solid substrate room temperature phosphorescence (SS-RTP) signal of some polyhalogenated derivatives of fluorescein (including eosin5-isothiocyanate) on a polyamide membrane can be enhanced obviously when an external inorganic salt was introduced into the system. The enhancement effect (RTP intensity and the ratio of signal to noise) of CaCl2 was much more notable than those of typical heavy atom perturbers in phosphorimetry, such as Pb(AC)(2) and Hg(NO3)(2), etc. According to that, a solid substrate room temperature phosphorescence immunoassay (SS-RTP-IA) with eosin5-isothiocyanate as a marker and CaCl2 as an enhancer for the determination of human complement 3 was developed. On a polyamide membrane, SS-RTP signals (λ(ex)/λ(em) = 532 nm/685 nm) of the immune complex are in linear correlation to the amount of human C3 in the range of 0.156-5.00 pg/spot (the sample volume was 0.4 μ L/spot, corresponding concentration was 0.391-12.5 ng/mL). The regression equation of working curve can be expressed as &UDelta; I-p (&UDelta; I-p = I-sample - I-blank) = 1.57 + 0.633 m(C3) (pg/spot), correlation coefficient r = 0.998. The detection limit calculated as 3 S-b/k is 0.12 pg/spot. The method was directly applied to the determination of human complement 3 in human serums with satisfactory results. These results proved sufficiently that eosin-ITC is a good phosphorescent marker, while CaCI2 was used as the enhancer. Obviously, the results will expand the application area of these luminescent molecules, and using CaCI2 instead of Pb(AC)(2) as a phosphorescence enhancer is more favorable for environment. © 2005 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/13347]
专题	清华大学
推荐引用方式 GB/T 7714	Wu, YL,Li, LD,Liu, JM,et al. The enhancement effect of CaCl2 on solid substrate room temperature phosphorescence of polyhalogenated derivatives of fluorescein and application in immunoassay[J],2010, 2010.
APA	Wu, YL,Li, LD,Liu, JM,&Zhu, GH.(2010).The enhancement effect of CaCl2 on solid substrate room temperature phosphorescence of polyhalogenated derivatives of fluorescein and application in immunoassay..
MLA	Wu, YL,et al."The enhancement effect of CaCl2 on solid substrate room temperature phosphorescence of polyhalogenated derivatives of fluorescein and application in immunoassay".(2010).

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