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	The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: A synchrotron X-ray diffraction study
	Chen, Lin ; Yu, Zhiwu ; Quinn, Peter J.
	2010-05-06 ; 2010-05-06
关键词	liquid-ordered phase bilayer electron density phosphatidylcholine cholesterol partition coefficient membrane raft DIFFERENTIAL SCANNING CALORIMETRY DETERGENT-SOLUBLE BILAYERS NUCLEAR-MAGNETIC-RESONANCE LIPID RAFTS MODEL MEMBRANES DIPALMITOYLPHOSPHATIDYLCHOLINE MIXTURES CONDENSED COMPLEXES LATERAL DIFFUSION TERNARY MIXTURES DOMAIN FORMATION Biochemistry & Molecular Biology Biophysics
中文摘要	The structure and composition of coexisting bilayer phases separated in binary mixtures of dipalmitoylphosphatidylcholine and cholesterol and ternary mixtures of equimolar proportions of dipalmitoyl- and dioleoylphosphatidycholines containing different proportions of cholesterol have been characterized by synchrotron X-ray diffraction methods. The liquid-ordered phase is distinguished from gel and fluid phases by a disordering of the hydrocarbon chains intermediate between the two phases as judged from the wide-angle X-ray scattering profiles. Electron density distribution calculated in coexisting bilayer phases shows that liquid-ordered phase is enriched in dipalmitoylphosphatidylcholine and cholesterol and a higher electron density in the methylene chain region of the bilayer ascribed to the location of the sterol ring of cholesterol. The ratio of the two constituents in the liquid-ordered phase is not constant because the stoichiometry is temperature-dependent as seen by respective changes in bilayer thickness over the range 20 degrees to 36 degrees C where coexisting phases are observed. Three coexisting phases were deconvolved in the ternary mixture at 20 degrees C. From an analysis of the ternary mixtures containing mole fractions of cholesterol from 0.09 to 0.15 it was found that the liquid-crystal and gel phases each contained about 10% of the cholesterol molecules and the liquid-ordered phase was comprised of 30% cholesterol molecules. (c) 2007 Elsevier B.V. All rights reserved.
语种	英语 ; 英语
出版者	ELSEVIER SCIENCE BV ; AMSTERDAM ; PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/12539]
专题	清华大学
推荐引用方式 GB/T 7714	Chen, Lin,Yu, Zhiwu,Quinn, Peter J.. The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: A synchrotron X-ray diffraction study[J],2010, 2010.
APA	Chen, Lin,Yu, Zhiwu,&Quinn, Peter J..(2010).The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: A synchrotron X-ray diffraction study..
MLA	Chen, Lin,et al."The partition of cholesterol between ordered and fluid bilayers of phosphatidylcholine: A synchrotron X-ray diffraction study".(2010).

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