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	Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide
	Zheng, Yufang ; Li, Haifang ; Guo, Zhihong ; Lin, Jin-Ming ; Cai, Zongwei
	2010-05-06 ; 2010-05-06
关键词	beta-elimination reaction chip-MS glycopeptide MS/MS O-GLYCOSYLATION SITES CAPILLARY-ELECTROPHORESIS MICROCHIP DEVICES DEAD VOLUME ELECTROSPRAY INTEGRATION IDENTIFICATION LOCALIZATION OPTIMIZATION SEPARATIONS Biochemical Research Methods Chemistry, Analytical
中文摘要	A novel approach using sheath flow chip-based CE coupled to a quadrupole TOF mass spectrometer was developed and applied to the analysis of an O-glycopeptide. The method provided good separation and sensitive analysis of the glycopeptide and its P-elimination product. The P-elimination reaction of the glycopeptide with ammonia or dimethylamine was adapted for changing the glycopeptide into deglycosylated form and for specifying the site of glycosylation. MS/MS analysis of the native peptide, glycopeptide and P-elimination product enabled the identification of the glycosylation site through the comparison of the fragmentations and peptide sequence analysis.
语种	英语 ; 英语
出版者	WILEY-V C H VERLAG GMBH ; WEINHEIM ; PO BOX 10 11 61, D-69451 WEINHEIM, GERMANY
内容类型	期刊论文
源URL	[http://hdl.handle.net/123456789/12365]
专题	清华大学
推荐引用方式 GB/T 7714	Zheng, Yufang,Li, Haifang,Guo, Zhihong,et al. Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide[J],2010, 2010.
APA	Zheng, Yufang,Li, Haifang,Guo, Zhihong,Lin, Jin-Ming,&Cai, Zongwei.(2010).Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide..
MLA	Zheng, Yufang,et al."Chip-based CE coupled to a quadrupole TOF mass spectrometer for the analysis of a glycopeptide".(2010).

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