Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate | |
Fan, Jinxin1,2; Luo, Jianquan1; Chen, Xiangrong1; Wan, Yinhua1 | |
刊名 | JOURNAL OF CHROMATOGRAPHY A |
2017-03-25 | |
卷号 | 1490页码:54-62 |
关键词 | Mussel-inspired Salt-tolerant Anion-exchange Membrane Adsorber Monoclonal Antibody Polishing Membrane Chromatography |
ISSN号 | 0021-9673 |
DOI | 10.1016/j.chroma.2017.02.016 |
文献子类 | Article |
英文摘要 | In this study, a polyvinylidene fluoride (PVDF) hydrophobic membrane with high mechanical property was used as substrate to prepare salt-tolerant anion-exchange (STAE) membrane adsorber. Effective hydrophilization and functionalization of PVDF membrane was realized via polydopamine (PDA) deposition, thus overcoming the drawbacks of hydrophobic substrates including poor water permeability, inert property as well as severe non-specific adsorption. The following polyallylamine (PAH) coupling was carried out at pH 10.0, where unprotonated amine groups on PAH chains were more prone to couple with PDA. This membrane adsorber could remain 75% of protein binding capacity when NaCl concentration increased from 0 to 150 mM, while its protein binding capacity was independent of flow rate from 10 to 100 membrane volume (MV)/min due to its high mechanical strength (tensile strength: 43.58 +/- 2.30 MPa). With 200 mM NaCI addition at pH 7.5, high purity (above 99%) and high recovery (almost 100%) of Immunoglobulin G (IgG) were obtained when using the STAE membrane adsorber to separate IgG/human serum albumin (HSA) mixture, being similar to that without NaCI at pH 6.0 (both under the flow rate of 10-100 MV/min). Finally, the reliable reusability was confirmed by five reuse cycles of protein binding and elution operations. In comparison with commercial membrane adsorber, the new membrane adsorber exhibited a better mechanical property, higher IgG polishing efficiency and reusability, while the protein binding capacity was lower due to less NH2 density on the membrane. The outcome of this work not only offers a facile and effective approach to prepare membrane adsorbers based on hydrophobic membranes, but also demonstrates great potential of this new designed STAE membrane adsorbers for efficient monoclonal antibody (mAb) polishing. (C) 2017 Elsevier B.V. All rights reserved. |
WOS关键词 | Affinity Membranes ; Hollow Fibers ; Purification ; Protein ; Chromatography ; Polydopamine ; Coatings ; Electrospun ; Clearance ; Platform |
WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
语种 | 英语 |
WOS记录号 | WOS:000397375600008 |
资助机构 | National High Technology Research and Development Program of China (863 Program)(2012AA021903 |
内容类型 | 期刊论文 |
源URL | [http://ir.ipe.ac.cn/handle/122111/22077] |
专题 | 过程工程研究所_生化工程国家重点实验室 |
作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, PR, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, PR, Peoples R China |
推荐引用方式 GB/T 7714 | Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,et al. Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate[J]. JOURNAL OF CHROMATOGRAPHY A,2017,1490:54-62. |
APA | Fan, Jinxin,Luo, Jianquan,Chen, Xiangrong,&Wan, Yinhua.(2017).Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate.JOURNAL OF CHROMATOGRAPHY A,1490,54-62. |
MLA | Fan, Jinxin,et al."Facile preparation of salt-tolerant anion-exchange membrane adsorber using hydrophobic membrane as substrate".JOURNAL OF CHROMATOGRAPHY A 1490(2017):54-62. |
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