Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum

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	Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum
	Liu, Boyan 1,2; Cao, Lili 1; Zhang, Lijun 1,2; Yuan, Xiaofan1 ; Zhao, Bing 1
刊名	MOLECULES
	2017
卷号	22 期号:1
关键词	Eupatorium adenophorum chlorogenic acid sesquiterpenes safety toxicity
ISSN号	1420-3049
英文摘要	Eupatorium adenophorum is widely distributed throughout the world's tropical and temperate regions. It has become a harmful weed of crops and natural environments. Its leaves contain bioactive compounds such as chlorogenic acid and may be used as feed additives. In this study, chlorogenic acid was extracted and separated from leaves of E. adenophorum. Three chlorogenic acid products were prepared with different purities of 6.11%, 22.17%, and 96.03%. Phytochemical analysis demonstrated that the main toxins of sesquiterpenes were almost completely removed in sample preparation procedure. The three products were evaluated for safety via in vitro and in vivo toxicological studies. All the products exhibited no cytotoxic effects at a dose of 400 mu g/mL in an in vitro cell viability assay. When administered in vivo at a single dose up to 1.5 g/kg bw, all three products caused no signs or symptoms of toxicity in mice. These results encourage further exploration of extracts from E. adenophorum in feed additive application.
WOS标题词	Science & Technology ; Physical Sciences
类目[WOS]	Chemistry, Organic
研究领域[WOS]	Chemistry
关键词[WOS]	IN-VITRO ; AGERATINA-ADENOPHORA ; COUNTERCURRENT CHROMATOGRAPHY ; ESSENTIAL OILS ; CAFFEIC ACID ; HEPG2 CELLS ; SEPARATION ; HEPATOTOXICITY ; CYTOTOXICITY ; PURIFICATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000395473500067
内容类型	期刊论文
源URL	[http://ir.ipe.ac.cn/handle/122111/21994]
专题	过程工程研究所_生化工程国家重点实验室
作者单位	1.Chinese Acad Sci, Inst Proc Engn, Div Biorefinery Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu, Boyan,Cao, Lili,Zhang, Lijun,et al. Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum[J]. MOLECULES,2017,22(1).
APA	Liu, Boyan,Cao, Lili,Zhang, Lijun,Yuan, Xiaofan,&Zhao, Bing.(2017).Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum.MOLECULES,22(1).
MLA	Liu, Boyan,et al."Preparation, Phytochemical Investigation, and Safety Evaluation of Chlorogenic Acid Products from Eupatorium adenophorum".MOLECULES 22.1(2017).