The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans

	The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans
	Dong, Yijie 1; Yu, Qilin 1; Chen, Yulu 1; Xu, Ning 3; Zhao, Qiang 1; Jia, Chang 1; Zhang, Bing 1; Zhang, Kai 1; Zhang, Biao 2; Xing, Laijun 1
刊名	INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
	2015-12-01
卷号	69 页码:41-51
关键词	Candida albicans Ccz1 Autophagy Oxidative stress Virulence
英文摘要	Autophagy plays a critical role in response to numerous cellular stresses, such as nutrient deprivation, hypoxia, starvation and organelle damage. The disruption of autophagy pathway affects multiple aspects of cellular stress response. Here we for the first time identified Ccz1 as an essential component for autophagy in Candida albicans. Our experiments demonstrated that loss of CCZ1 gene led to vacuolar fragmentation and disruption of the autophagy pathway. Our results also suggested that Ccz1 functioned in oxidative stress. In the Ccz1 Delta/Delta mutant, the levels of reactive oxidative species (ROS) sharply increased under H2O2 treatment. Further studies demonstrated that breakdown of the autophagic clearance pathway led to the accumulation of oxidative stress-damaged mitochondria, and consequently elevated cellular ROS levels in the ccz1 Delta/Delta. mutant. Furthermore, deletion of CCZ1 led to a significant defect in filamentous development at both 30 degrees C and 37 degrees C. The disruption of CCZ/ gene led to decreased capacity of macrophage killing and increased sensitivity to the macrophages. In addition, the ccz1 Delta/Delta mutant exhibited attenuated virulence and decreased fungal burdens in the mouse systemic infection model, indicating that CCZ1 might provide a promising target for antifungal drugs development. In summary, our findings provide new insights into the understanding of autophagy-related gene in C. albicans. (C) 2015 Elsevier Ltd. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]	Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]	VACUOLAR MEMBRANE PERMEABILIZATION ; SACCHAROMYCES-CEREVISIAE ; PROTEIN CCZ1P ; IRON ACQUISITION ; CELL-DEATH ; H+-ATPASE ; YEAST ; VIRULENCE ; MORPHOGENESIS ; MITOPHAGY
收录类别	SCI
语种	英语
WOS记录号	WOS:000367020800005
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2891]
专题	天津工业生物技术研究所_总体研究部_期刊论文
作者单位	1.Nankai Univ, Coll Life Sci, Dept Microbiol, Key Lab Mol Microbiol & Technol,Minist Educ, Tianjin 300071, Peoples R China 2.Tianjin Univ Tradit Chinese Med, Tianjin, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Dong, Yijie,Yu, Qilin,Chen, Yulu,et al. The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2015,69:41-51.
APA	Dong, Yijie.,Yu, Qilin.,Chen, Yulu.,Xu, Ning.,Zhao, Qiang.,...&Li, Mingchun.(2015).The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,69,41-51.
MLA	Dong, Yijie,et al."The Ccz1 mediates the autophagic clearance of damaged mitochondria in response to oxidative stress in Candida albicans".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 69(2015):41-51.