Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli

	Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli
	Bai, Wenqin 1,2,3; Tai, Yi-Shu 1; Wang, Jingyu 1; Wang, Jilong 1; Jambunathan, Pooja 1; Fox, Kevin J.1; Zhang, Kechun 1
刊名	METABOLIC ENGINEERING
	2016-11-01
卷号	38 页码:285-292
关键词	Escherichia coli Nonphosphorylative metabolism Dicarboxylic acid Mesaconate Pentose
英文摘要	Dicarboxylic acids are attractive biosynthetic targets due to their broad applications and their challenging manufacturing process from fossil fuel feedstock. Mesaconate is a branched, unsaturated dicarboxylic acid that can be used as a co-monomer to produce hydrogels and fire-retardant materials. In this study, we engineered nonphosphorylative metabolism to produce mesaconate from D-xylose and L-arabinose. This nonphosphorylative metabolism is orthogonal to the intrinsic pentose metabolism in Escherichia call and has fewer enzymatic steps and a higher theoretical yield to TCA cycle intermediates than the pentose phosphate pathway. Here mesaconate production was enabled from the D-xylose pathway and the L-arabinose pathway. To enhance the transportation of D-xylose and L-arabinose, pentose transporters were examined. We identified the pentose/proton symporter, AraE, as the most effective transporter for both D-xylose and L-arabinose in mesaconate production process. Further production optimization was achieved by operon screening and metabolic engineering. These efforts led to the engineered strains that produced 12.5 g/l and 13.2 g/l mesaconate after 48 h from 20 g/l of D-xylose and L-arabinose, respectively. Finally, the engineered strain overexpressing both L-arabinose and D-xylose operons produced 14.7 g/l mesaconate from a 1:1 D-xylose and L-arabinose mixture with a yield of 85% of the theoretical maximum. (0.87 g/g). This work demonstrates an effective system that converts pentoses into a value-added chemical, mesaconate, with promising titer, rate, and yield.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Biotechnology & Applied Microbiology
研究领域[WOS]	Biotechnology & Applied Microbiology
关键词[WOS]	CARBON CATABOLITE REPRESSION ; D-XYLOSE ; METHYLOBACTERIUM-EXTORQUENS ; PENTOSE OXIDATION ; SUCCINIC ACID ; PATHWAY ; FERMENTATION ; BACTERIA ; PURIFICATION ; EXPRESSION
收录类别	SCI
语种	英语
WOS记录号	WOS:000387984600028
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2291]
专题	天津工业生物技术研究所_总体研究部_期刊论文
作者单位	1.Univ Minnesota, Dept Chem Engn & Mat Sci, Minneapolis, MN 55455 USA 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 3.Shanxi Normal Univ, Coll Life Sci, Linfen 041004, Peoples R China
推荐引用方式 GB/T 7714	Bai, Wenqin,Tai, Yi-Shu,Wang, Jingyu,et al. Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli[J]. METABOLIC ENGINEERING,2016,38:285-292.
APA	Bai, Wenqin.,Tai, Yi-Shu.,Wang, Jingyu.,Wang, Jilong.,Jambunathan, Pooja.,...&Zhang, Kechun.(2016).Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli.METABOLIC ENGINEERING,38,285-292.
MLA	Bai, Wenqin,et al."Engineering nonphosphorylative metabolism to synthesize mesaconate from lignocellulosic sugars in Escherichia coli".METABOLIC ENGINEERING 38(2016):285-292.