Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli

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	Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli
	Yu, Shanshan 1,2,3; Yao, Peiyuan 2,3; Li, Jianjiong 2,3; Ren, Jie 2,3; Yuan, Jing 2,3; Feng, Jinhui 2,3; Wang, Min 1; Wu, Qiaqing 2,3; Zhu, Dunming 2,3
刊名	JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
	2016-07-01
卷号	129 页码:37-42
关键词	Nitrilase Immobilization 3-Hydroxypropionic acid Hydrolysis Enzyme catalysis
英文摘要	3-Hydroxypropionic acid (3-HP) is an important platform chemical for organic synthesis and high performance polymers. Despite a wealth of reports related to 3-HP biosynthesis in microorganisms, its industrial application still requires further research because of low titer and productivity. Herein an effective enzymatic method for the synthesis of 3-HP was achieved by using free or immobilized recombinant Escherichia coli BL21(DE3) cells harboring a nitrilase gene from environmental sample (NIT190). Under the optimal conditions (100 mmol/LTris-HCl buffer, pH 8.0, 30 degrees C), the maximum substrate concentration which could be completely hydrolyzed by using free cells within 24h was 4.5 mol/L (319.5 g/L). Furthermore, immobilization of the whole cells enhanced their substrate tolerance (up to 7.0 mol/L), stability, and reusability. The immobilized cells could be reused for up to 30 batches, and 70% of enzyme activity was retained after 74 batches in distilled water. The titer (184.7 g/L) and productivity (36.9 g/(Lh)) were obtained by isolation and purification of 3-HP from the first 30 batches. These results demonstrate that the immobilized cells have potential industrial application for the synthesis of 3-HP. (C) 2016 Elsevier B.V. All rights reserved.
WOS标题词	Science & Technology ; Life Sciences & Biomedicine ; Physical Sciences
类目[WOS]	Biochemistry & Molecular Biology ; Chemistry, Physical
研究领域[WOS]	Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]	GLYCEROL METABOLISM ; SACCHAROMYCES-CEREVISIAE ; LACTOBACILLUS-REUTERI ; NITRILASE ; TRANSFORMATION ; HYDROLYSIS ; PATHWAY ; INTERMEDIATE ; EFFICIENT
收录类别	SCI
语种	英语
WOS记录号	WOS:000377729700006
内容类型	期刊论文
源URL	[http://124.16.173.210/handle/834782/2905]
专题	天津工业生物技术研究所_生物催化与绿色化工朱敦明_期刊论文
作者单位	1.Tianjin Univ Sci & Technol, Coll Biotechnol, Minist Educ, Key Lab Ind Fermentat Microbiol, Tianjin 300457, Peoples R China 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, 32 Xi Qi Dao, Tianjin 300308, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin Engn Ctr Biocatalyt Technol, Tianjin Airport Econ Area, 32 Xi Qi Dao, Tianjin 300308, Peoples R China
推荐引用方式 GB/T 7714	Yu, Shanshan,Yao, Peiyuan,Li, Jianjiong,et al. Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli[J]. JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,2016,129:37-42.
APA	Yu, Shanshan.,Yao, Peiyuan.,Li, Jianjiong.,Ren, Jie.,Yuan, Jing.,...&Zhu, Dunming.(2016).Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli.JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC,129,37-42.
MLA	Yu, Shanshan,et al."Enzymatic synthesis of 3-hydroxypropionic acid at high productivity by using free or immobilized cells of recombinant Escherichia coli".JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC 129(2016):37-42.