Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides

CORC > 上海硅酸盐研究所 > 中国科学院上海硅酸盐研究所 > 高性能陶瓷和超微结构国家重点实验室 > 期刊论文

	Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides
	Zhao, Yiman 1; Zhang, Lingyi 1; Chu, Zhanying 1; Xiong, Zhichao 2; Zhang, Weibing 1
刊名	ANALYTICAL METHODS
	2017
卷号	9 期号:3 页码:443-449
英文摘要	For in-depth analysis of phosphorylated proteomics, the highly sensitive and selective capture of phosphopeptides from intricate biological samples is extremely significant. In this work, a late-model immobilized metal ion affinity chromatography (IMAC) material was fabricated and applied to enriching phosphopeptides from biological samples by abundant Ti4+ ions, which were chelated to crosslinked chitosan (CS) loaded on the surface of magnetic graphene oxide (GO) (denoted as mag GO-CS-Ti4+). The high amount of crosslinked CS endowed the IMAC material with highly hydrophilic properties and abundant active sites for Ti4+ ions on the surface. These characteristics ensure the great performance of mag GO-CS-Ti4+ in the selective enrichment of phosphopeptides from the tryptic digest of beta-casein with high selectivity (phosphopeptides to non-phosphopeptides at a molar ratio of 1 : 400), high sensitivity (0.5 fmol), large enrichment capacity (66.6 mg g(-1)), prominent phosphopeptide recovery (93.11%), and excellent reusability. What's more, mag GO-CS-Ti4+ was applied to capturing phosphopeptides from real biological samples, human serum from both healthy people and patients and nonfat milk. The results successfully manifested that the mag GO-CS-Ti4+ could be a great affinity material for the enrichment of low-abundant phosphopeptides from biological samples.
WOS标题词	Science & Technology ; Physical Sciences ; Life Sciences & Biomedicine ; Technology
类目[WOS]	Chemistry, Analytical ; Food Science & Technology ; Spectroscopy
研究领域[WOS]	Chemistry ; Food Science & Technology ; Spectroscopy
关键词[WOS]	METAL-ORGANIC FRAMEWORK ; MASS-SPECTROMETRY ; QUANTITATIVE PROTEOMICS ; FACILE SYNTHESIS ; NANOPARTICLES ; MICROSPHERES ; PHOSPHOPROTEOMICS ; SEPARATION ; CAPTURE ; PHOSPHORYLATION
收录类别	SCI
语种	英语
WOS记录号	WOS:000393102400010
内容类型	期刊论文
源URL	[http://ir.sic.ac.cn/handle/331005/23484]
专题	上海硅酸盐研究所_高性能陶瓷和超微结构国家重点实验室_期刊论文
作者单位	1.East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China
推荐引用方式 GB/T 7714	Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,et al. Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides[J]. ANALYTICAL METHODS,2017,9(3):443-449.
APA	Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,Xiong, Zhichao,&Zhang, Weibing.(2017).Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides.ANALYTICAL METHODS,9(3),443-449.
MLA	Zhao, Yiman,et al."Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides".ANALYTICAL METHODS 9.3(2017):443-449.