Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室

	Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation
	Zhao H 1; Liu F 1,2; Li CP[]3; Wu SL 3; Yang L[]3; Zheng YP[*]1,4
刊名	Nanoscale
	2017
卷号	9 期号:X 页码:e4272
通讯作者	zhangyp@mail.kiz.ac.cn ; long133109070@126.com, lcppp1974@sina.com
英文摘要	Alternative cleavage and polyadenylation (APA) is involved in several important biological processes in animals, e.g. cell growth and development, and cancer progression. The increasing data show that cancer cells are inclined to produce mRNA isoforms with a shortened 3'UTR undergoing APA. For example, the Dicer1 isoform with a shorter 3'untranslated region (3'UTR) was found to be overexpressed in some cancer cells, which may be used as a potential novel prognostic biomarker for cancer. In the present work, a novel electrochemical biosensor for ultrasensitive determination of Dicer1 was designed by using gold nanoparticles and p-sulfonated calix[6]arene functionalized reduced graphene oxide (Au@SCX6-rGO) as nanocarriers. The results showed that the expressions of the shorter 3'UTR (Dicer1-S) both in BT474 and SKBR3 were obviously higher than those of the longer Dicer1 (Dicer1-L) by the constructed biosensor, which agreed well with the result analyzed by the RT-qPCR method. The detection ranges of Dicer1-S and Dicer1-L were 10-14-10-9 M and 10-15-10-10 M. The LODs were 3.5 and 0.53 fM. The specificity of the proposed biosensor was also very high. For the first time, the expressional analysis of different 3'UTRs caused by APA was studied by an electrochemical method. Moreover, the use of a macrocyclic host for constructing an electrochemical/biosensing platform has rarely been reported. The proposed electrochemical sensing strategy is thus expected to provide a new method for determination of novel biomarkers and a novel method for fast and cheap analysis of APA.
收录类别	SCI
资助信息	This work was financially supported by the National Natural Science Foundation of China (21565029, 31260032, 31460038) and the Program for Excellent Young Talents (WX069051), Yunnan University.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/11037]
专题	昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_分子进化基因组学
作者单位	1.Laboratory for Conservation and Utilization of Bio-resource, Yunnan University, Kunming 650091, PR China 2.School of Life Science, Yunnan University, Kunming 650091, China 3.School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China 4.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, PR China
推荐引用方式 GB/T 7714	Zhao H,Liu F,Li CP[*],et al. Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation[J]. Nanoscale,2017,9(X):e4272.
APA	Zhao H,Liu F,Li CP[],Wu SL,Yang L[],&Zheng YP[*].(2017).Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation.Nanoscale,9(X),e4272.
MLA	Zhao H,et al."Ultrasensitive electrochemical detection of Dicer1 3’UTR for the fast analysis of alternative cleavage and polyadenylation".Nanoscale 9.X(2017):e4272.