Selective responses of human-gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured TiO2 | |
Wang, Xiao1,2; Lu, Tao3; Wen, Jin1,2; Xu, Lianyi1,2; Zeng, Deliang1,2; Wu, Qianju1,2; Cao, Lingyan1,2; Lin, Shuxian1,2; Liu, Xuanyong3; Jiang, Xinquan1,2 | |
刊名 | BIOMATERIALS |
2016-03-01 | |
卷号 | 83页码:207-218 |
关键词 | Carbon-fiber-reinforced polyetheretherketone Nanopores Focal adhesion Peri-implant soft tissue Antibacterial activity |
英文摘要 | The long-term success of dental implants relies not only on stable osseointegration but also on the integration of implant surfaces with surrounding soft tissues. In our previous work, titanium plasma immersion ion implantation (PIII) technique was applied to modify the carbon-fiber-reinforced polyetheretherketone (CFRPEEK) surface, constructing a unique multilevel TiO2 nanostructure thus enhancing certain osteogenic properties. However, the interactions between the modified surface and soft-tissue cells are still not clear. Here, we fully investigate the biological behaviors of human gingival fibroblasts (HGFs) and oral pathogens on the structured surface, which determine the early peri-implant soft tissue integration. Scanning electron microscopy (SEM) shows the formation of nanopores with TiO2 nanoparticles embedded on both the sidewall and bottom. In vitro studies including cell adhesion, viability assay, wound healing assay, real-time PCR, western blot and enzyme-linked immunosorbent assay (ELISA) disclose improved adhesion, migration, proliferation, and collagen secretion ability of HGFs on the modified CFRPEEK. Moreover, the structured surface exhibits sustainable antibacterial properties towards Streptococcus mutans, Fusobacterium nucleatum and Porphyromonas gingivalis. Our results reveal that the multilevel TiO2 nanostructures can selectively enhance soft tissue integration and inhibit bacterial reproduction, which will further support and broaden the adoption of CFRPEEK materials in dental fields. (C) 2016 Elsevier Ltd. All rights reserved. |
WOS标题词 | Science & Technology ; Technology |
类目[WOS] | Engineering, Biomedical ; Materials Science, Biomaterials |
研究领域[WOS] | Engineering ; Materials Science |
关键词[WOS] | OF-THE-LITERATURE ; DENTAL IMPLANTS ; SOFT-TISSUE ; IN-VITRO ; SURFACE CHARACTERISTICS ; STEM-CELLS ; TITANIUM ; ATTACHMENT ; BARRIER ; OSTEOGENESIS |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000371651700017 |
内容类型 | 期刊论文 |
源URL | [http://ir.sic.ac.cn/handle/331005/23150] |
专题 | 上海硅酸盐研究所_生物材料与组织工程研究中心_期刊论文 |
作者单位 | 1.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Dept Prosthodont, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Peoples Hosp 9, Oral Bioengn Lab,Shanghai Res Inst Stomatol,Shang, 639 Zhizaoju Rd, Shanghai 200011, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Xiao,Lu, Tao,Wen, Jin,et al. Selective responses of human-gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured TiO2[J]. BIOMATERIALS,2016,83:207-218. |
APA | Wang, Xiao.,Lu, Tao.,Wen, Jin.,Xu, Lianyi.,Zeng, Deliang.,...&Jiang, Xinquan.(2016).Selective responses of human-gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured TiO2.BIOMATERIALS,83,207-218. |
MLA | Wang, Xiao,et al."Selective responses of human-gingival fibroblasts and bacteria on carbon fiber reinforced polyetheretherketone with multilevel nanostructured TiO2".BIOMATERIALS 83(2016):207-218. |
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