Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 环境化学与生态毒理学国家重点实验室

	Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer
	Liu, Yingxiong; Zhao, Qiang
刊名	ANALYTICAL METHODS
	2016-01
卷号	8 期号:11 页码:2383-2390
英文摘要	Here we report a fluorescence anisotropy (FA) assay for D-vasopressin (D-Vas), an important active peptide, by using a tetramethylrhodamine (TMR) labeled 55-nt aptamer that contains 19 guanine (G) bases. This assay is based on a target-binding induced change of the intramolecular interaction between TMR and G base and the subsequent FA alteration of labeled TMR. We conjugated single TMR on different labeling positions of thymine (T) bases or terminals of the aptamer, obtaining 14 TMR-labeled aptamers. We screened the FA response of each TMR-labeled aptamer upon D-Vas addition. An aptamer probe with TMR labeled on the 34th base T in sequence exhibited sensitive and significant FA-decreasing responses upon the binding of D-Vas. Under optimized assay conditions this favorable TMR-labeled aptamer allowed direct FA detection of D-Vas ranging from 0.2 mu M to 200 mu M with good selectivity. This FA method also enabled the detection of D-Vas spiked in diluted serum and urine samples.
收录类别	SCI
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35857]
专题	生态环境研究中心_环境化学与生态毒理学国家重点实验室
推荐引用方式 GB/T 7714	Liu, Yingxiong,Zhao, Qiang. Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer[J]. ANALYTICAL METHODS,2016,8(11):2383-2390.
APA	Liu, Yingxiong,&Zhao, Qiang.(2016).Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer.ANALYTICAL METHODS,8(11),2383-2390.
MLA	Liu, Yingxiong,et al."Fluorescence anisotropy assay for D-vasopressin with a tetramethylrhodamine-labeled aptamer".ANALYTICAL METHODS 8.11(2016):2383-2390.