Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains

CORC > 生态环境研究中心 > 中国科学院生态环境研究中心 > 城市与区域生态国家重点实验室

	Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains
	Zhang, J.; Zhao, C. -Y.; Liu, J.; Song, R.; Du, Y. -X.; Li, J. -Z.; Sun, H. -Z.; Duan, G. -L.; Zhao, Q. -Z.
刊名	PLANT MOLECULAR BIOLOGY REPORTER
	2016-06
卷号	34 期号:3 页码:556-565
关键词	Sulfur Arsenic Rice grains Illumina sequencing Differentially expressed genes
英文摘要	Arsenic (As) contamination of rice grains affects millions of people worldwide. In this study, we found that sulfur application (20As+120S) decreased As concentration in rice grains by 44 % compared to grains without sulfur application (20As+0S). Importantly, sulfur application decreased arsenate [As(V)] and arsenite [As(III)] concentration in rice grains significantly, while there was no significant effect on dimethylarsenate (DMA) concentration. To elucidate the molecular basis of As accumulation in rice grains, we performed Illumina sequencing to acquire the differentially expressed genes induced by arsenate and sulfur treatments. By contrast with the control, the expression of 1,000 genes was found to be changed significantly, with 46 genes up-regulated and 954 genes down-regulated in grains grown in arsenate-contaminated soil (20As+0S). Between samples of control and arsenate together with sulfur treatment (20As+120S), 1,169 genes expressed significantly differently, with 16 genes up-regulated and 1,153 genes down-regulated. Sulfur application significantly changed the expression of genes involved in As metabolism in rice grains, significantly down-regulated phosphate transporter gene OsPT23 and aquaporin gene OsTIP4;2, while ABC transporter genes (OsABCG5, OsABCI7_2 and OsABC6) and phytochelatin synthase genes (OsPCS1, OsPCS3 and OsPCS13) were up-regulated. These results provide an insight into the molecular basis of how sulfur assimilation regulates As accumulation in rice grains.
内容类型	期刊论文
源URL	[http://ir.rcees.ac.cn/handle/311016/35607]
专题	生态环境研究中心_城市与区域生态国家重点实验室
推荐引用方式 GB/T 7714	Zhang, J.,Zhao, C. -Y.,Liu, J.,et al. Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains[J]. PLANT MOLECULAR BIOLOGY REPORTER,2016,34(3):556-565.
APA	Zhang, J..,Zhao, C. -Y..,Liu, J..,Song, R..,Du, Y. -X..,...&Zhao, Q. -Z..(2016).Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains.PLANT MOLECULAR BIOLOGY REPORTER,34(3),556-565.
MLA	Zhang, J.,et al."Influence of Sulfur on Transcription of Genes Involved in Arsenic Accumulation in Rice Grains".PLANT MOLECULAR BIOLOGY REPORTER 34.3(2016):556-565.