Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 裴仁军团队

	Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate
	Yang, J; Zhu, J; Pei, R(裴仁军); Oliver, JA; Landry, DW; Stojanovic, MN; Lin, Q
刊名	ANALYTICAL METHODS
	2016
卷号	8 期号:26
通讯作者	Lin, Q
英文摘要	We present a microfluidic aptamer-based biosensor for detection of low-molecular-weight biomarkers in patient samples. Using a microfluidic device that integrates aptamer-based specific analyte extraction, isocratic elution, and detection by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry, we demonstrate rapid, sensitive and label-free detection of arginine vasopressin (AVP) in human plasma ultrafiltrate. AVP molecules in complex matrices are specifically captured by an aptamer that is immobilized on microbeads via affinity binding in a microchamber. After the removal of unbound, contaminating molecules through washing, aptamer-AVP complexes are thermally disrupted via on-chip temperature control. Released AVP molecules are eluted with purified water and transferred to a separate microchamber, and deposited onto a single spot on a MALDI plate via repeated, piezoelectrically actuated ejection, which enriches AVP molecules over the spot area. This integrated on-chip sample processing enables the quantitative detection of low-abundance AVP by MALDI-TOF mass spectrometry in a rapid and label-free manner. Our experimental results show the detection of AVP in human plasma ultrafiltrate as low as physiologically relevant picomolar concentrations via aptamer-based selective preconcentration, demonstrating the potential of our approach as a rapid (similar to 1 h), sensitive clinical AVP assay.
关键词[WOS]	APTAMER-BASED BIOSENSORS ; IN-VITRO SELECTION ; LABEL-FREE ; AU NANOPARTICLES ; AFFINITY-CHROMATOGRAPHY ; ADENOSINE-TRIPHOSPHATE ; FAST-EVAPORATION ; SMALL MOLECULES ; BLOOD-SERUM ; RNA LIGANDS
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000379479000002
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4595]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_裴仁军团队
推荐引用方式 GB/T 7714	Yang, J,Zhu, J,Pei, R,et al. Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate[J]. ANALYTICAL METHODS,2016,8(26).
APA	Yang, J.,Zhu, J.,Pei, R.,Oliver, JA.,Landry, DW.,...&Lin, Q.(2016).Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate.ANALYTICAL METHODS,8(26).
MLA	Yang, J,et al."Integrated microfluidic aptasensor for mass spectrometric detection of vasopressin in human plasma ultrafiltrate".ANALYTICAL METHODS 8.26(2016).