Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 戴建武团队

	Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry
	Huang, CH; Yang, QL; Song, FY; Chen, N; Yao, B; Liao, XL; Zhang, ST; Chen, YY(陈艳艳); Jin, G
刊名	INTEGRATED FERROELECTRICS
	2016
卷号	171 期号:1
通讯作者	Huang, CH ; Chen, YY(陈艳艳) ; Jin, G
英文摘要	This study describes the development of alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carbohydrate 19-9(CA19-9) multiplex immunoassay by integrated microfluidic device based on imaging ellipsometry (IMBIE). Different strategies for AFP, CEA and CA 19-9 simultaneous immunoassay on the same aldehyde-substrate were developed. Unifying the reaction steps of CA19-9 for antigen direct detection, CEA for secondary antibody (rabbit-antiCEA) addition, AFP for secondary (rabbit antiAFP) and an enhancer (goat IgG) application, and comprehensively considering the dynamic ranges and detection sensitivities, we combined these three markers into multiplex analysis in serial format.The sensitivities and dynamic ranges for CA19-9, CEA and AFP were 10.0U/mL, 1.0 ng/mL, 1.0 ng/mL and 2.0-100.0 U/mL, 0.1-64.0 ng/mL, 1.0-128.0 ng/mL, respectively. The intra-assay and inter-assay (coefficient variations) CVs of CA19-9, CEA and AFP were between 1.6-8.9% and 2.1-7.8%. The specificities tested for both cross-reaction and heterogeneous proteins of CA19-9, CEA and AFP were not more than 6.7%. Quantitative detection of 49 sera from gastric patients demonstrated that the accuracy for gastric cancer diagnosis was 83.4%. The total detection time is only 60min for an automated process. The proposed assay has the potential for practical application.
关键词[WOS]	HEPATOCELLULAR-CARCINOMA ; VISUALIZATION ; DIAGNOSIS ; ANTIGENS ; MARKERS ; CANCER
收录类别	SCI
语种	英语
WOS记录号	WOS:000378406600009
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4849]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_戴建武团队
推荐引用方式 GB/T 7714	Huang, CH,Yang, QL,Song, FY,et al. Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry[J]. INTEGRATED FERROELECTRICS,2016,171(1).
APA	Huang, CH.,Yang, QL.,Song, FY.,Chen, N.,Yao, B.,...&Jin, G.(2016).Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry.INTEGRATED FERROELECTRICS,171(1).
MLA	Huang, CH,et al."Label-free multiplex immunoassay of AFP, CEA and CA19-9 by integrated microfluidic biosensor based on imaging ellipsometry".INTEGRATED FERROELECTRICS 171.1(2016).