Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold

CORC > 苏州纳米技术与纳米仿生研究所 > 中国科学院苏州纳米技术与纳米仿生研究所 > 纳米生物医学与安全研究部 > 程国胜团队

	Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold
	Jiang, ZY(姜自云); Song, Q(宋琴); Tang, ML(唐明亮); Yang, LY(杨令延); Cheng, YL(程一琳); Zhang, M; Xu, DS; Cheng, GS(程国胜)
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016
卷号	8 期号:38
通讯作者	Cheng, GS(程国胜)
英文摘要	One of the key challenges in engineering neural tissues for cell-based therapies is to develop a biocompatible scaffold material to direct neural stem cell (NSC) behaviors. One great advantage for a scaffold would be to induce NSC migration toward pathological sites during regeneration and repair. In particular, the inflammatory responses in the pathological zone, which are mainly mediated by microglia in the central nervous system, affect the repair capacity of NSCs through NSC migration. Recently, graphene was used as a neural interface and scaffold material, but few studies have addressed the relationship between microglia. and NSCs in a graphene culture system. In this study, we used a combination of immunofluorescence, Western blotting, :enzyme-linked immunosorbent assays, and scanning electron microscopy to investigate how conditioned medium (CM) produced from microglia grown on two-dimensional graphene (2D-G) films or three-dimensional graphene (3D-G) foams govern NSC migration. The results revealed that the CM produced by microglia grown in 3D-G cultures could promote neurosphere formation, facilitate NSC migration from the neurospheres, and increase single cell polarization by activating the stromal cell-derived factor 1 alpha (SDF-1 alpha)/CXC chemokine-receptor 4 (CXCR4) signaling pathway and enhancing cell adhesion on the substrate. By contrast, the 2D-G CM failed to achieve these results. Our study suggests the great potential of 3D-G as a neural scaffold for NSC-based therapy in tissue engineering and regenerative medicine.
关键词[WOS]	RAT SUBVENTRICULAR ZONE ; OSTEOGENIC DIFFERENTIATION ; STRIPE ASSAY ; BRAIN-INJURY ; OXIDE ; PHOSPHORYLATION ; PROLIFERATION ; GLIOBLASTOMA ; INFLAMMATION ; RECRUITMENT
收录类别	SCI ; EI
语种	英语
WOS记录号	WOS:000384518500015
内容类型	期刊论文
源URL	[http://ir.sinano.ac.cn/handle/332007/4835]
专题	苏州纳米技术与纳米仿生研究所_纳米生物医学与安全研究部_程国胜团队
推荐引用方式 GB/T 7714	Jiang, ZY,Song, Q,Tang, ML,et al. Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold[J]. ACS APPLIED MATERIALS & INTERFACES,2016,8(38).
APA	Jiang, ZY.,Song, Q.,Tang, ML.,Yang, LY.,Cheng, YL.,...&Cheng, GS.(2016).Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold.ACS APPLIED MATERIALS & INTERFACES,8(38).
MLA	Jiang, ZY,et al."Enhanced Migration of Neural Stem Cells by Microglia Grown on a Three-Dimensional Graphene Scaffold".ACS APPLIED MATERIALS & INTERFACES 8.38(2016).