Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging | |
Yang, Yanlong1; Yao, Baoli1; Lei, Ming1; Dan, Dan1; Li, Runze1; Van Horn, Mark2; Chen, Xun3,4; Li, Yang3,4; Ye, Tong3,4,5 | |
刊名 | plos one |
2016-12-20 | |
卷号 | 11期号:12 |
ISSN号 | 1932-6203 |
通讯作者 | yao, bl (reprint author), chinese acad sci, xian inst opt & precis mech, state key lab transient opt & photon, xian, peoples r china. |
产权排序 | 1 |
英文摘要 | bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (edf), which makes it possible to observe fast events volumetrically. however, the depth information is lost due to integration of fluorescence signals along the propagation direction. we describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3d) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. the depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases. |
学科主题 | multidisciplinary sciences |
WOS标题词 | science & technology |
类目[WOS] | multidisciplinary sciences |
研究领域[WOS] | science & technology - other topics |
关键词[WOS] | light-sheet microscopy ; fluorescence microscopy ; deep |
收录类别 | SCI |
语种 | 英语 |
WOS记录号 | WOS:000392842900083 |
内容类型 | 期刊论文 |
源URL | [http://ir.opt.ac.cn/handle/181661/28666] |
专题 | 西安光学精密机械研究所_瞬态光学技术国家重点实验室 |
作者单位 | 1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China 2.Med Univ South Carolina, Dept Radiol, Charleston, SC USA 3.Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA 4.Clemson Univ, COMSET, Clemson, SC 29634 USA 5.Med Univ South Carolina, Dept Regenerat Med & Cell Biol, Charleston, SC 29425 USA |
推荐引用方式 GB/T 7714 | Yang, Yanlong,Yao, Baoli,Lei, Ming,et al. Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging[J]. plos one,2016,11(12). |
APA | Yang, Yanlong.,Yao, Baoli.,Lei, Ming.,Dan, Dan.,Li, Runze.,...&Ye, Tong.(2016).Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging.plos one,11(12). |
MLA | Yang, Yanlong,et al."Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging".plos one 11.12(2016). |
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