Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging
Yang, Yanlong1; Yao, Baoli1; Lei, Ming1; Dan, Dan1; Li, Runze1; Van Horn, Mark2; Chen, Xun3,4; Li, Yang3,4; Ye, Tong3,4,5
刊名plos one
2016-12-20
卷号11期号:12
ISSN号1932-6203
通讯作者yao, bl (reprint author), chinese acad sci, xian inst opt & precis mech, state key lab transient opt & photon, xian, peoples r china.
产权排序1
英文摘要

bessel beams have been successfully used in two-photon laser scanning fluorescence microscopy to extend the depth of field (edf), which makes it possible to observe fast events volumetrically. however, the depth information is lost due to integration of fluorescence signals along the propagation direction. we describe the design and implementation of two-photon lasers scanning stereomicroscopy, which allows viewing dynamic processes in three-dimensional (3d) space stereoscopically in real-time with shutter glasses at the speed of 1.4 volumes per second. the depth information can be appreciated by human visual system or be recovered with correspondence algorithms for some cases.

学科主题multidisciplinary sciences
WOS标题词science & technology
类目[WOS]multidisciplinary sciences
研究领域[WOS]science & technology - other topics
关键词[WOS]light-sheet microscopy ; fluorescence microscopy ; deep
收录类别SCI
语种英语
WOS记录号WOS:000392842900083
内容类型期刊论文
源URL[http://ir.opt.ac.cn/handle/181661/28666]  
专题西安光学精密机械研究所_瞬态光学技术国家重点实验室
作者单位1.Chinese Acad Sci, Xian Inst Opt & Precis Mech, State Key Lab Transient Opt & Photon, Xian, Peoples R China
2.Med Univ South Carolina, Dept Radiol, Charleston, SC USA
3.Clemson Univ, Dept Bioengn, Clemson, SC 29634 USA
4.Clemson Univ, COMSET, Clemson, SC 29634 USA
5.Med Univ South Carolina, Dept Regenerat Med & Cell Biol, Charleston, SC 29425 USA
推荐引用方式
GB/T 7714
Yang, Yanlong,Yao, Baoli,Lei, Ming,et al. Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging[J]. plos one,2016,11(12).
APA Yang, Yanlong.,Yao, Baoli.,Lei, Ming.,Dan, Dan.,Li, Runze.,...&Ye, Tong.(2016).Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging.plos one,11(12).
MLA Yang, Yanlong,et al."Two-Photon Laser Scanning Stereomicroscopy for Fast Volumetric Imaging".plos one 11.12(2016).
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