Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs

	Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs
	Huma T[*]1,2,4; Hu XT 1,3; Shahab M 2; Wang ZB 1,3; Ma YY 3; Willden A 3; Rizak J 1
刊名	Neuroscience
	2017
期号	**页码:In Press
关键词	Lyon ES kisspeptin GnRH neural stem cells proliferation
通讯作者	tanzeelhuma@gmail.com ; wangzb@mail.kiz.ac.cn
英文摘要	Embryonic stem cells (ESCs) have enormous potential as novel cell-based therapies, but their effectiveness depends on stem cell differentiation and specific signaling regulators, which remain poorly understood. In this study, a kisspeptin peptide (KP-10) was used at different dosages to determine whether rhesus macaque-derived tau GFP-Lyon ES cells underwent kisspeptin-specific neuronal differentiation. It was found that KP-10 exhibited an anti-proliferative effect on the cells and led to morphological changes and cellular differentiation consistent with neuronal stem cell (NSC) development. The cells differentiated into Gonadotrophin Releasing Hormone (GnRH) neuronal-like cell types in response to the KP-10 treatment. There has been a previously observed connection between kisspeptin signaling, GnRH neurons and their dysfunction found in congenital disorders like idiopathic hypogonadotropic hypogonadism (IHH). Although therapeutics are a still a far-off goal, the formation and development of GnRH-positive neuronal-like cells following the application of KP-10 to Lyon NSC cells opens the door for future NSC-based therapies to treat specific reproductive disorders.
收录类别	SCI
资助信息	This study was supported by research funds 645 from a TWAS-CAS visiting fellowship awarded to Ms. Tanzeel Huma, a Pilot Academy Strategic Technology Project 647 (XDB02020005) and the National Natural Science Foundation 648 of China (31271167).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10979]
专题	昆明动物研究所_认知障碍病理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室昆明动物研究所_神经系统编码
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China 2.Laboratory of Reproductive Neuroendocrinology, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad, Pakistan 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, PR China 4.Institute of Molecular Biology and Biotechnology, University of Lahore, Lahore, Pakistan
推荐引用方式 GB/T 7714	Huma T[],Hu XT,Shahab M,et al. Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs[J]. Neuroscience,2017(*):In Press.
APA	Huma T[].,Hu XT.,Shahab M.,Wang ZB.,Ma YY.,...&Rizak J.(2017).Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs.Neuroscience(*),In Press.
MLA	Huma T[],et al."Kisspeptin-10 treatment generated specific GnRH expression in cells differentiated from rhesus monkey derived Lyon NSCs".Neuroscience .*(2017):In Press.