CD317 Promotes the survival of cancer cells through apoptosis-inducing factor

	CD317 Promotes the survival of cancer cells through apoptosis-inducing factor
	Xin Li; Guizhong Zhang; Qian Chen; Yingxue Lin; Junxin Li; Qinggguo Ruan; Youhai Chen; Guang Yu; Xiaochun Wan
刊名	JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
	2016
英文摘要	Background: Low nutrient environment is a major obstacle to solid tumor growth. However, many tumors have developed adaptive mechanisms to circumvent the requirement for exogenous growth factors. Methods: Here we used siRNA interference or plasmid transfection techniques to knockdown or enhance CD317 expression respectively, in mammalian cancer cells, and subjected these CD317-manipulated cells to serum deprivation to study the role of CD317 on stress-induced apoptosis and the underlying mechanism. Results: We report that CD317, an innate immune gene overexpressed in human cancers, protected cancer cells against serum deprivation-induced apoptosis. In tumor cells, loss of CD317 markedly enhanced their susceptibility to serum deprivation-induced apoptosis with no effect on autophagy or caspase activation, indicating an autophagy-and caspase-independent mechanism of CD317 function. Importantly, CD317 knockdown in serum-deprived tumor cells impaired mitochondria function and subsequently promoted apoptosis-inducing factor (AIF) release and nuclear translocation but had little effect on mitochondrial and cytoplasmic distributions of cytochrome C, a pro-apoptotic factor released from mitochondria that initiates caspase processing in response to death stimuli. Furthermore, overexpression of CD317 in HEK293T cells inhibits serum deprivation-induced apoptosis as well as the release and nuclear accumulation of AIF. Conclusion: Our data suggest that CD317 functions as an anti-apoptotic factor through the mitochondria-AIF axis in malnourished condition and may serve as a potential drug target for cancer therapy.
收录类别	SCI
原文出处	https://jeccr.biomedcentral.com/articles/10.1186/s13046-016-0391-2
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/10728]
专题	深圳先进技术研究院_医药所
作者单位	JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
推荐引用方式 GB/T 7714	Xin Li,Guizhong Zhang,Qian Chen,et al. CD317 Promotes the survival of cancer cells through apoptosis-inducing factor[J]. JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH,2016.
APA	Xin Li.,Guizhong Zhang.,Qian Chen.,Yingxue Lin.,Junxin Li.,...&Xiaochun Wan.(2016).CD317 Promotes the survival of cancer cells through apoptosis-inducing factor.JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH.
MLA	Xin Li,et al."CD317 Promotes the survival of cancer cells through apoptosis-inducing factor".JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH (2016).