Steroid-Associated Hip Joint Collapse in Bipedal Emus

	Steroid-Associated Hip Joint Collapse in Bipedal Emus
	Li-Zhen Zheng; Zhong Liu; Jiang Peng; Yi-Xin He; Xin-Hui Xie; Chi-Wai Man; Le Huang; Xin-Luan Wang; Daniel Tik-Pui Fong; Ge Zhang
刊名	PLOS ONE
	2013
英文摘要	In this study we established a bipedal animal model of steroid-associated hip joint collapse in emus for testing potential treatment protocols to be developed for prevention of steroid-associated joint collapse in preclinical settings. Five adult male emus were treated with a steroid-associated osteonecrosis (SAON) induction protocol using combination of pulsed lipopolysaccharide (LPS) and methylprednisolone (MPS). Additional three emus were used as normal control. Post-induction, emu gait was observed, magnetic resonance imaging (MRI) was performed, and blood was collected for routine examination, including testing blood coagulation and lipid metabolism. Emus were sacrificed at week 24 post-induction, bilateral femora were collected for micro-computed tomography (micro-CT) and histological analysis. Asymmetric limping gait and abnormal MRI signals were found in steroid-treated emus. SAON was found in all emus with a joint collapse incidence of 70%. The percentage of neutrophils (Neut %) and parameters on lipid metabolism significantly increased after induction. Micro-CT revealed structure deterioration of subchondral trabecular bone. Histomorphometry showed larger fat cell fraction and size, thinning of subchondral plate and cartilage layer, smaller osteoblast perimeter percentage and less blood vessels distributed at collapsed region in SAON group as compared with the normal controls. Scanning electron microscope (SEM) showed poor mineral matrix and more osteo-lacunae outline in the collapsed region in SAON group. The combination of pulsed LPS and MPS developed in the current study was safe and effective to induce SAON and deterioration of subchondral bone in bipedal emus with subsequent femoral head collapse, a typical clinical feature observed in patients under pulsed steroid treatment. In conclusion, bipedal emus could be used as an effective preclinical experimental model to evaluate potential treatment protocols to be developed for prevention of ON-induced hip joint collapse in patients.
收录类别	SCI
原文出处	http://www.plosone.org/article/Authors/info:doi/10.1371/journal.pone.0076797
语种	英语
WOS记录号	WOS:000326032600011
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/4907]
专题	深圳先进技术研究院_医工所
作者单位	PLOS ONE
推荐引用方式 GB/T 7714	Li-Zhen Zheng,Zhong Liu,Jiang Peng,et al. Steroid-Associated Hip Joint Collapse in Bipedal Emus[J]. PLOS ONE,2013.
APA	Li-Zhen Zheng.,Zhong Liu.,Jiang Peng.,Yi-Xin He.,Xin-Hui Xie.,...&Ling Qin.(2013).Steroid-Associated Hip Joint Collapse in Bipedal Emus.PLOS ONE.
MLA	Li-Zhen Zheng,et al."Steroid-Associated Hip Joint Collapse in Bipedal Emus".PLOS ONE (2013).