ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells

	ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells
	Xiao Yiren; Jiang Zhiwu; Li Yin; Ye Wei; Jia Bei; Zhang Minjie; Xu Yan; Wu Donghai; Lai Liangxue; Chen Yaoyu
刊名	HAEMATOLOGICA
	2015
英文摘要	Successful expansion of hematopoietic stem cells would benefit the use of hematopoietic stem cell transplants in the clinic. Several angiopoietin-like proteins, including angiopoietin-like 7, can support the activity of hematopoietic stem cells. However, effects of ANGPTL7 on human hematopoietic stem cells and the downstream signaling cascade activated by ANGPTL7 are poorly understood. Here, we established a human hematopoietic stem and progenitor cell-supportive mouse fetal liver cell line that specifically expressed the Angptl7 protein. Furthermore, we found ANGPTL7 is capable of stimulating human hematopoietic stem and progenitor cell expansion and increasing the repopulation activities of human hematopoietic progenitors in xenografts. RNA-sequencing analysis showed that ANGPTL7 activated the expression of CXCR4, HOXB4 and Wnt downstream targets in human hematopoietic progenitors. In addition, chemical manipulation of Wnt signaling diminished the effects of ANGPTL7 on human hematopoietic stem and progenitor cells in culture. In summary, we identify the secreted growth factor ANGPTL7 as a regulator of both human hematopoietic stem and progenitor cell expansion and regeneration.
收录类别	SCI
原文出处	http://www.haematologica.org/content/100/5/585.full-text.pdf+html
语种	英语
内容类型	期刊论文
源URL	[http://ir.siat.ac.cn:8080/handle/172644/9158]
专题	深圳先进技术研究院_其他
作者单位	HAEMATOLOGICA
推荐引用方式 GB/T 7714	Xiao Yiren,Jiang Zhiwu,Li Yin,et al. ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells[J]. HAEMATOLOGICA,2015.
APA	Xiao Yiren.,Jiang Zhiwu.,Li Yin.,Ye Wei.,Jia Bei.,...&DuanqingLi Peng.(2015).ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells.HAEMATOLOGICA.
MLA	Xiao Yiren,et al."ANGPTL7 regulates the expansion and repopulation of human hematopoietic stem and progenitor cells".HAEMATOLOGICA (2015).