Genetic Polymorphisms of the CASP8 Gene Promoter May Not Be Associated with Colorectal Cancer in Han Chinese from Southwest China

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 重大疾病机理的遗传学

	Genetic Polymorphisms of the CASP8 Gene Promoter May Not Be Associated with Colorectal Cancer in Han Chinese from Southwest China
	Xiao MS 1; Chang L 2; Li WL 3; Du YS 2; Pan Y 2; Zhang DF 1,4; Wen Y 2; Luo J 2; Li XY[]2; Yao YG[]1
刊名	PLOS ONE
	2013
卷号	8 期号:7 页码:e67577
通讯作者	lixiaoyan-0808@163.com ; ygyaozh@gmail.com
合作状况	其它
英文摘要	Purpose: Caspase 8 (CASP8) plays a critical role in the apoptotic pathway and aberrant regulation of this pathway causes many diseases including cancers. Genetic variants rs3834129 (CTTACT/-) and rs3769821 (T/C) in the promoter region of the CASP8 gene were documented to be associated with multiple solid cancers and non-Hodgkin's lymphoma (NHL), respectively, despite of some controversies. We aimed to discern potential association of these two variants and rs113686495 (CTGTCATT/2), as well as CASP8 mRNA and protein expression levels with colorectal cancer (CRC) in Han Chinese. Methods: We genotyped CASP8 genetic variants in 305 CRC patients and 342 healthy individuals from Kunming, Southwest China. Expression levels of CASP8 mRNA and protein were quantified in paired cancerous and paracancerous normal tissues by using real-time quantitative PCR and western blot, respectively. We compared the frequencies of alleles, genotypes, and haplotypes between the cases and controls. Correlation of CASP8 mRNA and protein expression levels in paired cancerous and paracancerous normal tissues from patients with different genotypes and clinical expression were also evaluated. Results: There was no association of the CASP8 genetic variants with CRC in our case-control study. The CASP8 gene mRNA expression levels in cancerous and paracancerous normal tissues were similar and there was no significant difference between subjects with different genotypes and clinical features. However, we found that CASP8 protein level was significantly lower in cancerous tissues than in paired paracancerous normal tissues. Conclusions: Our results suggest that the three CASP8 genetic variants may not be associated with CRC risk in Han Chinese from southwest China. Aberrant CASP8 protein expression may play a role in the pathogenesis of CRC.
收录类别	SCI
资助信息	This study was supported by the Top Talents Program of Yunnan Province (2009CI119), National Natural Science Foundation of China (30925021) and Chinese Academy of Sciences.
语种	英语
WOS记录号	WOS:000321341000060
公开日期	2013-08-28
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7602]
专题	昆明动物研究所_重大疾病机理的遗传学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Kunming, China 2.Department of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, China 3.Department of Oncology, The First Affiliated Hospital of Kunming Medical University, Kunming, China 4.University of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Xiao MS,Chang L,Li WL,et al. Genetic Polymorphisms of the CASP8 Gene Promoter May Not Be Associated with Colorectal Cancer in Han Chinese from Southwest China[J]. PLOS ONE,2013,8(7):e67577.
APA	Xiao MS.,Chang L.,Li WL.,Du YS.,Pan Y.,...&Yao YG[*].(2013).Genetic Polymorphisms of the CASP8 Gene Promoter May Not Be Associated with Colorectal Cancer in Han Chinese from Southwest China.PLOS ONE,8(7),e67577.
MLA	Xiao MS,et al."Genetic Polymorphisms of the CASP8 Gene Promoter May Not Be Associated with Colorectal Cancer in Han Chinese from Southwest China".PLOS ONE 8.7(2013):e67577.