Increased Levels of b-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 肿瘤信号转导

	Increased Levels of b-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2
	Xu SX 1,2; Yang ZZ[*]1; Zhang JY 1; Jiang YX 1; Chen YB 3; Li HJ 4; Liu XF 1; Xu D 1; Chen YJ 1; Yang YH 1
刊名	DNA AND CELL BIOLOGY
	2014
卷号	31 期号:1 页码:1-9
通讯作者	yangzuozhang@163.com
合作状况	其它
英文摘要	To determine the expression of beta-catenin, lymphoid enhancer-binding protein-1 (LEF-1), and heparanase-1 (HPA-1) and to evaluate these proteins' potential prognostic values in malignant acral melanoma without mutations in BRAF exons 11 and 15 and NRAS exons 1 and 2, specimens from 90 patients with wild-type BRAF and NRAS were assessed and analyzed by immunohistochemistry and western blotting. The positive expression of beta-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 was observed in 36 (72%), 31 (62%), and 32 (64%) of the detected acral melanomas, respectively. The expression of beta-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 was not correlated with gender, age, or diseased body parts (p>0.05), but was significantly positively correlated with the tumor node metastasis (TNM) stage and metastasis (correlation=0.406 and 0.716, 0.397 and 0.582, 0.353 and 0.579; p=0.040 and 0.0001, 0.0040 and 0.0001, 0.0120 and 0.0001, respectively). We also observed that the increased expression of beta-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 was significantly correlated with decreased survival and poor prognosis (p=0.001, 0.010, and 0.023, respectively). A multifactorial analysis using Cox's regression model revealed that beta-catenin, lymphoid enhancer-binding protein-1, heparanase-1, and the TNM stage were all independent factors in malignant melanoma (risk ratios were 7.294, 5.550, 5.622, and 4.794; p-values were 0.007, 0.018, 0.018, and 0.029, respectively). This study may provide the basis for the use of beta-catenin, lymphoid enhancer-binding protein-1, and heparanase-1 as novel targets in the treatment of malignant invasion and metastasis in acral melanoma cancer. The expression of beta-catenin, LEF-1, and HPA-1 was assessed and compared in malignant melanoma with that of peritumoral tissue and benign nevus in the patients with negative mutations in BRAF exons 11 and 15 and NRAS exons 1 and 2. The study may provide the basis for beta-catenin, LEF-1, and HPA-1 as new targets in the treatment of malignant invasion and metastasis in melanoma cancer.
收录类别	SCI
资助信息	This research was supported, in part, by grants (no. 81260322/H1606, no. 81372322/H1606 and no. 81460440/ H1606) from the National Natural Science Foundation of China, a grant (no. 2012FB163) from the Natural Science Foundation of Yunnan Province, a grant (no. 2011FB201) from the Joint Special Funds for the Department of Science and Technology of Yunnan Province-Kunming Medical University, and a grant (no. D-201242) from the specialty fund of high- level talents medical personnel training of Yunnan province.
语种	英语
公开日期	2015-06-08
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8343]
专题	昆明动物研究所_肿瘤信号转导昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Department of Orthopedics, The Third Affiliated Hospital of Kunming Medical University, Tumor Hospital of Yunnan Province, Kunming, People’s Republic of China 2.Department of Orthopedics, The First People’s Hospital of Dali, Dali, People’s Republic of China 3.Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, People’s Republic of China 4.Institute of Medical Biology, Chinese Academy of Medical Science, Kunming, People’s Republic of China
推荐引用方式 GB/T 7714	Xu SX,Yang ZZ[*],Zhang JY,et al. Increased Levels of b-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2[J]. DNA AND CELL BIOLOGY,2014,31(1):1-9.
APA	Xu SX.,Yang ZZ[*].,Zhang JY.,Jiang YX.,Chen YB.,...&Xia JF.(2014).Increased Levels of b-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2.DNA AND CELL BIOLOGY,31(1),1-9.
MLA	Xu SX,et al."Increased Levels of b-catenin, LEF-1, and HPA-1 Correlate with Poor Prognosis for Acral Melanoma with Negative BRAF and NRAS Mutation in BRAF Exons 11 and 15 and NRAS Exons 1 and 2".DNA AND CELL BIOLOGY 31.1(2014):1-9.