γ-氨基丁酸受体和甘氨酸受体在大鼠丙泊酚麻醉中的作用 | |
谢玉波1; 徐林2; 刘敬臣1 | |
刊名 | 临床麻醉学杂志 |
2006 | |
卷号 | 22期号:9页码:691-693 |
关键词 | 兴奋性突触后电流 γ-氨基丁酸 甘氨酸 受体 丙泊酚 |
其他题名 | The role of GABA receptor and glycine receptor in propofol anesthesia |
合作状况 | 其它 |
中文摘要 | 目的研究丙泊酚对大鼠海马CA1区电刺激诱发兴奋性突触后电流(EPSC)的影响,分析γ-氨基丁酸(GABA)受体和甘氨酸受体在丙泊酚麻醉中的作用。方法断头法分离wistar大鼠(13~19d)海马半脑,切出400μm厚度的海马脑片,全细胞膜片钳技术记录CA1区锥体神经元EPSC。80张脑片分为八组:脂肪乳剂组,50μmol/L丙泊酚组,100μmol/L丙泊酚组,200μmol/L丙泊酚组,SR95531组,士的宁组,SR95531+100μmol/L丙泊酚组,士的宁+100μmol/L丙泊酚组,每组10张。SR95531+100μmol/L丙泊酚组和士的宁+100μmol/L丙泊酚组先在循环液中加入10μmol/LSR95531或4μmol/L士的宁预孵脑片30min。八组均记录基础EPSC10min,然后加入不同药物,继续记录EPSC40min。膜钳制电压为-70mV。结果脂肪乳剂、SR95531和士的宁对EP-SC幅值无影响;丙泊酚呈剂量依赖性的抑制EPSC幅值,50、100、200μmol/L丙泊酚最大抑制EPSC幅值为14.4%、52.3%、67.8%;SR95531+100μmol/L丙泊酚组加入丙泊酚后,EPSC幅值基本无改变;士的宁+100μmol/L丙泊酚组加入丙泊酚后,EPSC幅值仍然下降,最大抑制程度为34.7%。结论丙泊酚主要通过增强GABAA受体功能使兴奋性突触活动降低,甘氨酸受体在其中起到协同和调节作用。 |
英文摘要 | To investigate the effects of propofol on the whole-cell excitatory posts— ynaptic current(EPSC)in hippocampal CAl pyramidal neurons and analyze the role of GABAA receptor and glycine receptor in propofol anesthesia.Methods Wistar rats(13—19 d)were killed by cervical dislocation and hippocampal slices(400 Urn)were prepared.EPSCs were recorded by whole-eell patchclamp technique.The slices(n一80)were divided into eight groups.After 10 min baseline of EPSCs was recorded,different drugs were administered in perfusion for another 40 min.In the gabazine with 100 tlmol/L propofol group and the strychnine with 100 t_£mol/L propofol group,hippocampal slices were incubated with gabazine or strychnine for 30 rain before recording.The holding potential was 一70 mV.Results Intralipid,SR95531 and strychnine did not change the amplitude of EPSC,but 50, 100 and 200 pmol/L propofol reduced EPSC by 14.4%,52.3%and 67.8%,respectively.After hipp— ocampal slices were incubated with gabazine,100 umol/L propofol did not decrease EPSC.However。 after hippocampal slices were incubated with strychnine,100/utmol/L propofol still reduced the ampli— tude of EPSC by 34.7%.Conclusion Propofol could potentiate the function of GABAAreceptor and inhibit excitatory transmission.Glycine receptor may regulate the action of GABAA receptor in the CAl of hippocampus. |
收录类别 | 其他 |
资助信息 | 国家自然科学基金(39930080);广西青年基金(0542054)资助项目. |
语种 | 中文 |
公开日期 | 2010-08-09 |
内容类型 | 期刊论文 |
源URL | [http://159.226.149.42:8088/handle/152453/1075] |
专题 | 昆明动物研究所_学习记忆的分子神经机制 |
作者单位 | 1.广西医科大学第一附属医院麻醉科,广西南宁530021 2.中国科学院昆明动物研究所,昆明650223 |
推荐引用方式 GB/T 7714 | 谢玉波,徐林,刘敬臣. γ-氨基丁酸受体和甘氨酸受体在大鼠丙泊酚麻醉中的作用[J]. 临床麻醉学杂志,2006,22(9):691-693. |
APA | 谢玉波,徐林,&刘敬臣.(2006).γ-氨基丁酸受体和甘氨酸受体在大鼠丙泊酚麻醉中的作用.临床麻醉学杂志,22(9),691-693. |
MLA | 谢玉波,et al."γ-氨基丁酸受体和甘氨酸受体在大鼠丙泊酚麻醉中的作用".临床麻醉学杂志 22.9(2006):691-693. |
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