Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system

	Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system
	Chen XJ 1,3; Li TQ 1,2; Li XM 1,3; Xie YH 3; Guo XY 2,3; Ji SH 2; Niu YY 2; Yu Y 1,3; Ding CH 1,2,3; Ji WZ 2
刊名	REPRODUCTIVE BIOMEDICINE ONLINE
	2009
卷号	19 期号:3 页码:426-433
关键词	embryonic stem cells G5 supplement hepatocyte growth factor neural development neural differentiation
通讯作者	qzhou@ioz.ac.cn
合作状况	其它
英文摘要	A simple monoculture system. combined with a chemically defined medium containing hepatocyte growth factor (HGF) and G5 supplement, was used to induce rhesus monkey embryonic stem cells (rESC) directly into neuroepithelial (NE) cells. Under these conditions, the generation of NE cells did not require the formation of embryoid bodies or co-culture with other cell types. The NE cells could further develop to generate neurons, astrocytes and oligodendrocytes. These results demonstrate a simple approach to obtain enriched and expandable populations of neural progenitors. Importantly, unlike other systems, the neural progenitors obtained using this approach may possess the potential to differentiate into various regional neural cells. Finally, the results suggest that the time-dependent shift in the differentiation potential of the rESC-derived neural progenitors in vitro reflects the developmental events that occur during neurogenesis in vivo. Thus, this system can be used to study the mechanisms of cell fate specification during non-human primate neurogenesis.
收录类别	SCI
资助信息	This work was supported by research grants from Major State Basic Development Program (No. 2006CB701501, 2006CB701505, and 2007CB947702).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10475]
专题	昆明动物研究所_生殖与发育生物学昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, China 100101 2.Department of Reproduction and Development, Kunming Institute of Zoology and Kunming Primate Research Centre, the Chinese Academy of Sciences, Kunming, Yunnan, China 650223 3.Graduate University of Chinese Academy of Sciences, Beijing, China
推荐引用方式 GB/T 7714	Chen XJ,Li TQ,Li XM,et al. Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system[J]. REPRODUCTIVE BIOMEDICINE ONLINE,2009,19(3):426-433.
APA	Chen XJ.,Li TQ.,Li XM.,Xie YH.,Guo XY.,...&Yao RQ.(2009).Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system.REPRODUCTIVE BIOMEDICINE ONLINE,19(3),426-433.
MLA	Chen XJ,et al."Neural progenitors derived from monkey embryonic stem cells in a simple monoculture system".REPRODUCTIVE BIOMEDICINE ONLINE 19.3(2009):426-433.