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题名	1、蜜蜂嗅觉学习记忆应用基础研究 2、吗啡和胆碱能拮抗剂联合给药对小鼠Y迷宫空间记忆提取及活动性的影响
作者	何静
学位类别	硕士
答辩日期	2008-06
授予单位	中国科学院研究生院
授予地点	北京
导师	马原野
关键词	蜜蜂 学习记忆 嗅觉条件化 伸喙反应 气味探测 提取 吗啡 东莨菪碱 阿托品 Y迷宫
其他题名	Fundamental studies towards the application of the olfactory conditioning of honey bee (Apis mellifera) / Effect of co-administration of morphine and cholinergic antagonists on Y-maze spatial recognition memory retrieval and locomotor activity in mice
学位专业	细胞生物学
中文摘要	一、蜜蜂嗅觉学习记忆应用基础研究 特殊气味的探测在刑侦工作中意义重大，常用的警犬探测和仪器分析都有其局限之处。蜜蜂嗅觉灵敏，且学习记忆能力突出，具有为刑侦工作所用的潜力。基于此，我们希望通过训练蜜蜂将其对糖水奖励的伸喙反应与指定气味建立条件反射的原理，配合适当的训练方法，达到利用蜜蜂探测危险气味的目的。 在实验中，我们首先比较了不同喂养方式的蜜蜂在气味学习中的差别。由于低浓度气味无法直接使蜜蜂建立条件化，我们采用了逐渐降低气味浓度的方法，成功训练蜜蜂对低浓度(3.6×10-7) 醋酸气味建立了条件反射。结果如下： 1)自然放养与人工孵化两种不同喂养方式的蜜蜂，各两组，分别学习醋酸CS+/薄荷CS-，或柠檬CS+/薄荷CS-的气味配对。以“获得(CS+)，巩固(CS-/CS+ CS+/CS- CS-/CS+)，检测，干净空气假阳性检测”的顺序操作。结果显示自然放养蜜蜂对醋酸气味没有偏好（第一次给醋酸气味伸喙率：6%），学习醋酸气味能力较低（24小时后检测正确率：66%， n=25），相对应，该类蜜蜂对柠檬气味显示出明显偏好(第一次给柠檬气味伸喙率：41%，P< 0.01)，而学习效果（检测正确率：50%，n=20）与醋酸组相近(P>0.05)。人工孵化的蜜蜂对醋酸气味学习能力较自然放养蜜蜂大大提高（检测正确率：96%, n=32， P<0.01），同时对柠檬的学习结果（检测正确率：80%, n=32）也明显提高(0.01
英文摘要	Part1. Fundamental studies towards the application of the olfactory conditioning of honey bee (Apis mellifera) Specific odor detection plays an important role in scout for policemen. In order to compensate the limits of the police dog and the detecting devices, honey bees were considered for the potential odor detection ability according to their outstanding olfactory conditioning performance. In the first experiment, we studied the difference between the honey bees from two different adopted ways in olfactory conditioning abilities. Then, we managed to train honey bees to learn Ac odor at the extreme low concentration (3.6×10-7). More details see below. 1) Two ways for adopting honey bees; 1. Bees naturally developed in the comb, and then were caught at the entrance of the nest for experiment, or 2. Bees emerged in an artificially well controlled chest, and were adopted in a cage for 7 days. In the experiment, we trained the bees from the two adopting ways to study Ac CS+/ mint CS-, or lemon CS+/ mint CS-, thus together 4 groups were conducted. Each group went through the same manipulation order; “acquiring (CS+), consolidation (CS-/CS+ CS+/CS- CS-/CS+), test, and clean air false-positive test”. We found 1) the odor preference was only observed in the naturally developed bees towards lemon (pre-conditioning PER% to lemon: 41%, other 3 groups’ PER% to CS+ were around 10% at the beginning of training which was consistent to the spontaneous level). 2) Artificially adopted groups showed higher correct% (CS+/CS- rightly reacted bees’ number / group’s totality×100%) than the naturally developed bees. (Ac+/mint-: artificially adopted bees 96% n= 32, naturally developed bees 66% n=25, P<0.01; lemon+/mint-: artificially adopted bees 80% n= 32, naturally developed bees 50% n=20, 0.01
语种	中文
公开日期	2010-10-15
内容类型	学位论文
源URL	[http://159.226.149.42:8088/handle/152453/6278]
专题	昆明动物研究所_认知障碍病理学
推荐引用方式 GB/T 7714	何静. 1、蜜蜂嗅觉学习记忆应用基础研究 2、吗啡和胆碱能拮抗剂联合给药对小鼠Y迷宫空间记忆提取及活动性的影响[D]. 北京. 中国科学院研究生院. 2008.

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