Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme

	Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme
	Qu JG 1,2; Rizak JD 2,3; Fan YD 4; Guo XX 5; Li JJ 6; Huma T 2,7; Ma YY[*]2
刊名	HUMAN CELL
	2014
卷号	27 期号:3 页码:129-136
关键词	Left prefrontal lobe Glioblastoma multiforme Gene transcription Cell line
通讯作者	yuanma0716@vip.sina.com
合作状况	其它
英文摘要	This paper outlines the establishment of a new and stable cell line, designated GBM-HSF, from a malignant glioblastoma multiforme (GBM) removed from a 65-year-old Chinese woman. This cell line has been grown for 1 year without disruption and has been passaged over 50 times. The cells were adherently cultured in RPMI-1640 media with 10 % fetal bovine serum supplementation. Cells displayed spindle and polygonal morphology, and displayed multi-layered growth without evidence of contact inhibition. The cell line had a high growth rate with a doubling time of 51 h. The cells were able to grow without adhering to the culture plates, and 4.5 % of the total cells formed colonies in soft agar. The cell line has also been found to form tumors in nude mice and to be of a highly invasive nature. The cells were also partially characterized with RT-PCR. The RT-PCR revealed that Nestin, beta-tubulin III, Map2, Klf4, Oct4, Sox2, Nanog, and CD26 were positively transcribed, whereas GFAP, Rex1, and CD133 were negatively transcribed in this cell line. These results suggest that the GBM-HSF cell line will provide a good model to study the properties of cancer stem cells and metastasis. It will also facilitate more detailed molecular and cellular studies of GBM cell division and pathology.
收录类别	SCI
语种	英语
WOS记录号	WOS:000339341200005
公开日期	2014-10-16
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/8067]
专题	昆明动物研究所_认知障碍病理学昆明动物研究所_神经系统编码
作者单位	1.University of Science and Technology of China, Hefei, Anhui 230026, People’s Republic of China 2.State Key Laboratory of Brain and Cognitive Sciences, Kunming Institute of Zoology, Chinese Academy of Sciences, #32 Jiao Chang Dong Lu, Kunming, Yunnan 650223, People’s Republic of China 3.University of the Chinese Academy of Science, Beijing 100101, People’s Republic of China 4.Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University (Yunnan Province Tumor Hospital), Kunming, Yunnan 650118, People’s Republic of China 5.Beijing Key Laboratory of Functional Food from Plant Resources, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, People’s Republic of China 6.CAS-Max Planck Junior Scientist Group, State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, People’s Republic of China 7.Reproductive Neuroendocrinology Laboratory, Department of Animal Sciences, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan
推荐引用方式 GB/T 7714	Qu JG,Rizak JD,Fan YD,et al. Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme[J]. HUMAN CELL,2014,27(3):129-136.
APA	Qu JG.,Rizak JD.,Fan YD.,Guo XX.,Li JJ.,...&Ma YY[*].(2014).Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme.HUMAN CELL,27(3),129-136.
MLA	Qu JG,et al."Establishment and partial characterization of a human tumor cell line, GBM-HSF, from a glioblastoma multiforme".HUMAN CELL 27.3(2014):129-136.