Whole blood transcriptional profiling comparison between different milk yield of Chinese Holstein cows using RNA-seq data

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	Whole blood transcriptional profiling comparison between different milk yield of Chinese Holstein cows using RNA-seq data
	Bai X 1; Zheng ZQ 1,2; Zhang WG[]1,5; Liu B 1,2,3; Ji XY 1,2; Bai YS[]4
刊名	BMC GENOMICS
	2016
卷号	17 期号:增刊7 页码:e512
关键词	Whole blood Immune response Milk yield Differentially expressed genes Dairy cattle
通讯作者	Yongsheng.Bai@indstate.edu
英文摘要	Background: The objective of this research was to investigate the variation of gene expression in the blood transcriptome profile of Chinese Holstein cows associated to the milk yield traits. Results: We used RNA-seq to generate the bovine transcriptome from the blood of 23 lactating Chinese Holstein cows with extremely high and low milk yield. A total of 100 differentially expressed genes (DEGs) (p < 0.05, FDR < 0. 05) were revealed between the high and low groups. Gene ontology (GO) analysis demonstrated that the 100 DEGs were enriched in specific biological processes with regard to defense response, immune response, inflammatory response, icosanoid metabolic process, and fatty acid metabolic process (p < 0.05). The KEGG pathway analysis with 100 DEGs revealed that the most statistically-significant metabolic pathway was related with Toll-like receptor signaling pathway (p < 0.05). The expression level of four selected DEGs was analyzed by qRT-PCR, and the results indicated that the expression patterns were consistent with the deep sequencing results by RNA-Seq. Furthermore, alternative splicing analysis of 100 DEGs demonstrated that there were different splicing pattern between high and low yielders. The alternative 3' splicing site was the major splicing pattern detected in high yielders. However, in low yielders the major type was exon skipping. Conclusion: This study provides a non-invasive method to identify the DEGs in cattle blood using RNA-seq for milk yield. The revealed 100 DEGs between Holstein cows with extremely high and low milk yield, and immunological pathway are likely involved in milk yield trait. Finally, this study allowed us to explore associations between immune traits and production traits related to milk production.
收录类别	SCI
资助信息	This work was supported by National Natural Science Foundation of China (No.30960246), and “National Science and Technology Support Plan” of China (No.2011BAD28B05).
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10762]
专题	昆明动物研究所_其他
作者单位	1.College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China 2.Institute of ATCG Nei Mongol Bio-Information, Hohhot 010020, China 3.Nei Mongol BioNew Technology Co.Ltd, Hohhot 010018, China 4.Department of Biology, Indiana State University, Terre Haute, IN 47809, U.S.A 5.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China
推荐引用方式 GB/T 7714	Bai X,Zheng ZQ,Zhang WG[*],et al. Whole blood transcriptional profiling comparison between different milk yield of Chinese Holstein cows using RNA-seq data[J]. BMC GENOMICS,2016,17(增刊7):e512.
APA	Bai X,Zheng ZQ,Zhang WG[],Liu B,Ji XY,&Bai YS[].(2016).Whole blood transcriptional profiling comparison between different milk yield of Chinese Holstein cows using RNA-seq data.BMC GENOMICS,17(增刊7),e512.
MLA	Bai X,et al."Whole blood transcriptional profiling comparison between different milk yield of Chinese Holstein cows using RNA-seq data".BMC GENOMICS 17.增刊7(2016):e512.