Thiazolidine reacts with thioreactive biomolecules

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 离子通道药物研发中心

	Thiazolidine reacts with thioreactive biomolecules
	Su DY 1,2; Nian Y 1; Zhou M 1,4; Yang J[]1,5; Wang S[]1,2; Zhang FL 1; Hu JS 1,2; Cui JM 1,3
刊名	Free Radical Biology and Medicine
	2017
卷号	104 期号:X 页码:272-279
关键词	Glutathione TRPA1 channel Thiazolidine
通讯作者	jy160@columbia.edu, jianyang@mail.kiz.ac.cn ; wangshu@mail.kiz.ac.cn
英文摘要	The thiazolidine ring is a biologically active chemical structure and is associated with many pharmacological activities. However, the biological molecules that can interact with the thiazolidine ring are not known. We show that thiazolidine causes sustained activation of the TRPA1 channel and chemically reacts with glutathione, and the chemical reactivity of thiazolidine ring is required for TRPA1 activation. Reducing agents reverse thiazolidine-induced TRPA1 activation, and mutagenesis studies show that nucleophilic cysteine residues in TRPA1 are critical, suggesting an activation mechanism involving thioreactive chemical reactions. In vivo studies show that thiazolidine induces acute pain and inflammation in mouse and these responses are specifically dependent on TRPA1. These results indicate that thiazolidine compounds can chemically react with biological molecules containing nucleophilic cysteines, thereby exerting biological activities.
收录类别	SCI
资助信息	This work was supported by grants from the National Natural Science Foundation of China (81302865), Yunnan Applied Basic Research Projects (2013FB074), Youth Innovation Promotion Association of the Chinese Academy of Sciences, Key Research Program of the Chinese Academy of Sciences (KJZD-EW-L03) and West Light Foundation of the Chinese Academy of Sciences to Shu Wang; grants from the National Basic Research Program of China (2014CB910301), Top Talents Program of Yunnan Province (2011HA012), High-level Overseas Talents of Yunnan Province, Yunnan Major Science and Technology Project (2015ZJ002) and the National Natural Science Foundation of China (31370821) to Jian Yang; Grants from the High-level Overseas Talents of Yunnan Province and the National Basic Research Program of China (2014CB910301) to Ming Zhou; and a Grant from High-level Overseas Talents of Yunnan Province to Jianmin Cui.
语种	英语
内容类型	期刊论文
源URL	[http://159.226.149.26:8080/handle/152453/10831]
专题	昆明动物研究所_离子通道药物研发中心昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Sciences/Key Laboratory of Bioactive Peptides of Yunnan Province, and Ion Channel Research and Drug Development Center, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Kunming 650204, China 3.Department of Biomedical Engineering, Center for the Investigation of Membrane Excitability Disorders, Cardiac Bioelectricity and Arrhythmia Center, Washington University, St. Louis, MO 63130, USA 4.Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine, Houston, TX 77030, US 5.Department of Biological Sciences, Columbia University, New York, NY 10027, USA
推荐引用方式 GB/T 7714	Su DY,Nian Y,Zhou M,et al. Thiazolidine reacts with thioreactive biomolecules[J]. Free Radical Biology and Medicine,2017,104(X):272-279.
APA	Su DY.,Nian Y.,Zhou M.,Yang J[].,Wang S[].,...&Cui JM.(2017).Thiazolidine reacts with thioreactive biomolecules.Free Radical Biology and Medicine,104(X),272-279.
MLA	Su DY,et al."Thiazolidine reacts with thioreactive biomolecules".Free Radical Biology and Medicine 104.X(2017):272-279.