Molecular Evolution of the Primate alpha-/theta-Defensin Multigene Family

	Molecular Evolution of the Primate alpha-/theta-Defensin Multigene Family
	Cheng DQ 1,2; Li Y 1,3; Huang JF[*]1,4
刊名	PLOS ONE
	2014
卷号	9 期号:5 页码:e97425
通讯作者	huangjf@mail.kiz.ac.cn
合作状况	其它
英文摘要	The primate alpha-/theta-defensin multigene family encodes versatile endogenous cationic and amphipathic peptides that have broad-spectrum antibacterial, antifungal and antiviral activity. Although previous studies have reported that alpha-/theta-defensin (DEFA/DEFT) genes are under birth-and-death evolution with frequent duplication and rapid evolution, the phylogenetic relationships of the primate DEFA/DEFT genes; the genetic bases for the existence of similar antimicrobial spectra among closely related species; and the evolutionary processes involved in the emergence of cyclic theta-defensins in Old World monkeys and their subsequent loss of function in humans, chimpanzees and gorillas require further investigation. In this study, the DEFA/DEFT gene repertoires from primate and treeshrew were collected, followed by detailed phylogenetic, sequence and structure, selection pressure and comparative genomics analyses. All treeshrew, prosimian and simian DEFA/DEFT genes are grouped into two major clades, which are tissue-specific for enteric and myeloid defensins in simians. The simian enteric and myeloid alpha-defensins are classified into six functional gene clusters with diverged sequences, variable structures, altered functional constraints and different selection pressures, which likely reflect the antimicrobial spectra among closely related species. Species-specific duplication or pseudogenization within each simian cluster implies that the antimicrobial spectrum is ever-shifting, most likely challenged by the ever-changing pathogen environment. The DEFT evolved from the myeloid DEFA8. The prosegment of theta-defensin is detected with adaptive changes coevolving with the new protein fold of mature peptide, coincident with the importance of the prosegment for the correct folding of the mature peptide. Lastly, a less-is-hitchhiking hypothesis was proposed as a possible explanation for the expansion of pseudogene DEFTP and the loss of functional DEFT, where the gain or loss of the hitchhiker is determined by its adjacent driver gene during the birth-and-death evolutionary process.
收录类别	SCI
资助信息	This work was supported by the National Natural Science Foundation of China (Grant No. 31123005) and Chinese Academy of Sciences (Grant No. 2007211311091) for JFH, and the ‘‘100-Talent Program’’ in Sichuan and National Natural Science Foundation of China (Grant No. 30600066) for YL.
语种	英语
WOS记录号	WOS:000336653300111
公开日期	2014-06-03
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7852]
专题	昆明动物研究所_结构生物信息学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, China 2.Kunming College of Life Science, University of Chinese Academy of Sciences, Beijing, China 3.Institute of Animal Genetics and Breeding, College of Animal Science and Technology, Sichuan Agricultural University, Yaan, China 4.Kunming Institute of Zoology-Chinese University of Hongkong Joint Research Center for Bio-resources and Human Disease Mechanisms, Kunming, China
推荐引用方式 GB/T 7714	Cheng DQ,Li Y,Huang JF[*]. Molecular Evolution of the Primate alpha-/theta-Defensin Multigene Family[J]. PLOS ONE,2014,9(5):e97425.
APA	Cheng DQ,Li Y,&Huang JF[*].(2014).Molecular Evolution of the Primate alpha-/theta-Defensin Multigene Family.PLOS ONE,9(5),e97425.
MLA	Cheng DQ,et al."Molecular Evolution of the Primate alpha-/theta-Defensin Multigene Family".PLOS ONE 9.5(2014):e97425.