Identification and characterization of insect-specific proteins by genome data analysis

	Identification and characterization of insect-specific proteins by genome data analysis
	Zhang G 1,2,3; Wang HS 1; Shi JJ 2; Wang XL 2; Zheng HK 2; Wong GKS 2; Clark T 4; Wang W 3; Wang J 2,5; Kang L[*]1
刊名	BMC GENOMICS
	2007
卷号	8 期号:X 页码:e93
ISSN号	1471-2164
通讯作者	lkang@ioz.ac.cn
合作状况	其它
英文摘要	Background: Insects constitute the vast majority of known species with their importance including biodiversity, agricultural, and human health concerns. It is likely that the successful adaptation of the Insecta clade depends on specific components in its proteome that give rise to specialized features. However, proteome determination is an intensive undertaking. Here we present results from a computational method that uses genome analysis to characterize insect and eukaryote proteomes as an approximation complementary to experimental approaches. Results: Homologs in common to Drosophila melanogaster, Anopheles gambiae, Bombyx mori, Tribolium castaneum, and Apis mellifera were compared to the complete genomes of three non-insect eukaryotes ( opisthokonts) Homo sapiens, Caenorhabditis elegans and Saccharomyces cerevisiae. This operation yielded 154 groups of orthologous proteins in Drosophila to be insect-specific homologs; 466 groups were determined to be common to eukaryotes ( represented by three opisthokonts). ESTs from the hemimetabolous insect Locust migratoria were also considered in order to approximate their corresponding genes in the insect-specific homologs. Stress and stimulus response proteins were found to constitute a higher fraction in the insect-specific homologs than in the homologs common to eukaryotes. Conclusion: The significant representation of stress response and stimulus response proteins in proteins determined to be insect-specific, along with specific cuticle and pheromone/odorant binding proteins, suggest that communication and adaptation to environments may distinguish insect evolution relative to other eukaryotes. The tendency for low Ka/Ks ratios in the insect-specific protein set suggests purifying selection pressure. The generally larger number of paralogs in the insect-specific proteins may indicate adaptation to environment changes. Instances in our insect-specific protein set have been arrived at through experiments reported in the literature, supporting the accuracy of our approach.
收录类别	SCI
资助信息	This project was supported by the National Basic Research Program of China (No:2006CB102002), Chinese Academy of Sciences (GJHZ0518), Ministry of Science and Technology under program CNGI-04-15-7A, National Natural Science Foundation of China (90208019; 90403130; 30221004), and China National Grid. Other support came from Danish Platform for Integrative Biology, Ole Rømer grants from the Danish Natu- ral Science Research Council and National Science Foundation (DBI 0217241).
原文出处	2007893.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4827]
专题	昆明动物研究所_基因起源组昆明动物研究所_细胞与分子进化重点实验室
作者单位	1.State Key Laboratory of Genetic Resource and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Beijing Institute of Genomics of Chinese Academy of Sciences, Beijing Genomics Institute, Beijing 101300, China 3.CAS- Max Plank Junior Research Group, Key Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Science (CAS), Kunming, Yunnan 650223, China 4.Department of Electrical Engineering and Computer Science, The University of Kansas, 2001 Eaton Hall, Lawrence, KS 66044, USA 5.Department of Biochemistry and Molecular Biology, University of Southern Denmark, DK-5230, Odense M, Denmark
推荐引用方式 GB/T 7714	Zhang G,Wang HS,Shi JJ,et al. Identification and characterization of insect-specific proteins by genome data analysis[J]. BMC GENOMICS,2007,8(X):e93.
APA	Zhang G.,Wang HS.,Shi JJ.,Wang XL.,Zheng HK.,...&Kang L[*].(2007).Identification and characterization of insect-specific proteins by genome data analysis.BMC GENOMICS,8(X),e93.
MLA	Zhang G,et al."Identification and characterization of insect-specific proteins by genome data analysis".BMC GENOMICS 8.X(2007):e93.