Allosteric regulation of the calcium-sensing receptor in obese individuals

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子人类学

	Allosteric regulation of the calcium-sensing receptor in obese individuals
	He YH 1,2; Perry B 3; Bi MX 1; Sun H 4; Zhao TT 5; Li Y[]1; Sun CH[]1
刊名	INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
	2013
卷号	32 期号:2 页码:511-518
关键词	calcium-sensing recepor obesity cytokine calcium amino acids
通讯作者	liying_helen@163.com ; sun2002changhao@yahoo.com
合作状况	其它
英文摘要	We have previously reported that the calcium-sensing receptor (CaSR) plays an important role in modulating lipid metabolism under low calcium conditions. The aim of this study was to identify possible regulators of CaSR and the mechanisms of action of CaSR in obese individuals. Subcutaneous fat samples were obtained from 10 obese and 10 non-obese males undergoing elective abdominal surgery. Visceral fat pads were obtained from 12 obese and 12 non-obese male rats. Serum lipid, tumor necrosis factor-alpha (TNF-alpha) and interleukin-6 (IL-6) concentrations, as well as the gene and protein expression of CaSR in the white adipose tissue of obese subjects and rats were determined. Serum total calcium, vitamin D, parathyroid hormone (PTH) and amino acid levels in human subjects were measured. Intracellular calcium and cyclic adenosine monophosphate (cAMP) levels in rat adipocytes were measured by laser scanning confocal microscopy and ELISA, respectively. The results revealed that serum triglyceride (TG), total cholesterol (TC), low density lipoprotein-cholesterol (LDL-C), TNF-alpha, IL-6 and PTH levels were significantly higher in the obese individuals versus the controls. By contrast, serum vitamin D and amino acid concentrations were lower in the obese individuals versus the controls. In addition, intracellular calcium levels were higher, while cAMP levels were lower in the obese rat adipocytes compared with the control group. However, the gene and protein expression of CaSR in white adipose tissue did not differ between the obese groups and the controls. Thus, these results suggest that CaSR functions not through its expression, but rather through allosteric regulation in obese individuals.
收录类别	SCI
资助信息	This study was funded by grants from the National Natural Science Fund of China key project (no. 81130049) and the Program for New Century Excellent Talents in University of China (no. NCET-10-0148).
语种	英语
WOS记录号	WOS:000322370800032
公开日期	2013-08-30
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7620]
专题	昆明动物研究所_分子人类学昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.National Key Discipline of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, Harbin, Heilongjiang 150081 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, P.R. China 3.Aquatic and Crop Resource Development, Life Sciences Branch, National Research Council Canada, Charlottetown, Prince Edward Island C1A 4P3, Canada 4.Emergency Department, The Second Affiliated Hospital, Harbin Medical University, Harbin, Heilongjiang 150001, P.R. China 5.Department of Psychology, University of Toronto, Toronto, Ontario M1C 1A4, Canada
推荐引用方式 GB/T 7714	He YH,Perry B,Bi MX,et al. Allosteric regulation of the calcium-sensing receptor in obese individuals[J]. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,2013,32(2):511-518.
APA	He YH.,Perry B.,Bi MX.,Sun H.,Zhao TT.,...&Sun CH[*].(2013).Allosteric regulation of the calcium-sensing receptor in obese individuals.INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE,32(2),511-518.
MLA	He YH,et al."Allosteric regulation of the calcium-sensing receptor in obese individuals".INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 32.2(2013):511-518.