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题名	猕猴属三个近缘物种的阴道微生物组比较研究
作者	朱林
学位类别	博士
答辩日期	2015-04
授予单位	中国科学院研究生院
授予地点	北京
导师	郑永唐
关键词	非人灵长类动物 微生物组 时间动态变异性 月经周期 网络分析
其他题名	Comparative Study of Vaginal Microbiome in Three Closely Related Species of Macaca
中文摘要	猕猴属三个近缘物种的阴道微生物组比较研究 博士研究生：朱 林（细胞生物学） 导师：郑永唐 研究员 复杂和动态变化的宿主阴道微生态系统是由宿主与阴道微生物组长期共同进化而形成的，并对宿主的健康和疾病有十分重要的作用。全面理解阴道微生物组的组成和时间动态变异性受制于诸如培养法，克隆文库等低分辨率的分子技术和横断面研究的内在局限性。随着高通量测序技术的迅猛发展和应用，阴道微生物群落的复杂性和动态性超出了人们之前的想象。 迄今为止，在受限于研究物种，时间动力学分辨率以及元数据获取的条件下，我们对于阴道微生物组的结构和时间动态变异性的理解已有长足进展。然而，我们对于宿主遗传背景是否能够影响阴道微生物组的时间动态变异性以及宿主生理因子的时间动态变化是否是影响阴道微生物组正常的时间动态变异性的因素这两个重要的问题还知之甚少。 本论文基于16S rRNA基因的454焦磷酸测序技术，得到了来源于六只食蟹猴，两只北平顶猴以及六只中国猕猴共计387个时间点的阴道微生物组数据集以及收集了包括阴道pH，体温，雌二醇和孕酮的元数据集。研究结果提供了猕猴属三个近缘物种的阴道微生物组的结构和高分辨率的时间动力学的参考目录。研究结果表明，宿主的遗传背景能够影响阴道微生物组的结构和时间动态变异性。进一步的网络分析发现，生理因子与阴道微生物组相互作用的关联网络的全局和局部特性在三个物种间具有不同的特点，揭示了生理因子的时间动态变化对阴道微生物组时间动态变异性的影响。最后，我们发现月经周期的不同分期能够影响阴道微生物组的群落多样性。综上所述，本论文的研究结果表明宿主的遗传背景和生理因子对复杂和动态变化的阴道微生态系统具有重要的影响。
英文摘要	Comparative Study of Vaginal Microbiome in Three Closely Related Species of Macaca Ph D. Candidate: Lin Zhu(Cell Biology) Directed by Prof. Yong-Tang Zheng The complex and dynamic host vaginal microbial ecosystem is commonly thought to have been shaped by long-term co-evolutionary processes between the vaginal microbiome and its host and critical to both health and disease of host. Comprehensive understanding the composition and temporal variability of vaginal microbiome remain poorly elucidated due to inherent limitations of culture-dependent methods, low-resolution molecular methods such as clone library, cross-sectional study designs. As high-throughput sequencing-based techniques for microbiome analysis have been developed and used, vaginal microbiome has been revealed as considerably more complex and dynamic. To date, despite the significant advances in understanding of structure and temporal variability of vaginal microbiome in a limited context in terms of host species, resolution of temporal dynamics, and metadata, little is known about two critical questions: whether host genetics contribute to aspects of composition and temporal variability of vaginal microbiome and whether dynamic changes of physiological factors levels contribute to the normal variability in vaginal microbiome through time. Here, we provide a comprehensive catalog of structure and high-resolution dynamics of vaginal microbiome using datasets of 454-pyrosequencing of 16S rRNA genes and metadata including vaginal pH, body temperature, estradiol, and progesterone, covering six cynomolgus, two northern pig-tailed, and six Chinese rhesus macaques over 387 timepoints. Strikingly, we demonstrate host genetics play a predominant role in influencing the structure and temporal dynamics of vaginal microbiome. Next, network analyses reveal the globe and local properties of correlation networks constructed from vaginal microbiome and physiological factors vary widely among three Macaca species, elucidating how the normal variability in vaginal microbiome through time may arise in response to dynamic changes of physiological factors levels. Finally, we uncover phases of the menstrual cycle could influence diversity of vaginal microbiome. Overall, our results highlight the influence of host genetics and physiological factors on complex and dynamic vaginal microbial ecosystem.
语种	中文
内容类型	学位论文
源URL	[http://159.226.149.26:8080/handle/152453/10143]
专题	昆明动物研究所_分子免疫药理学
推荐引用方式 GB/T 7714	朱林. 猕猴属三个近缘物种的阴道微生物组比较研究[D]. 北京. 中国科学院研究生院. 2015.

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