Identification and characterization of a novel splice variant of rhesus macaque MHC IA

CORC > 昆明动物研究所 > 昆明动物研究所 > 动物模型与人类重大疾病机理重点实验室 > 分子免疫药理学

	Identification and characterization of a novel splice variant of rhesus macaque MHC IA
	Dai ZX 1,2; Zhang GH 1; Zhang XH 1,2; Zheng YT[*]1
刊名	MOLECULAR IMMUNOLOGY
	2013
卷号	53 期号:3 页码:206-213
关键词	Rhesus macaque Alternative splicing MHC IA Splice variant cis-Interaction Heterodimer
通讯作者	zhengyt@mail.kiz.ac.cn
合作状况	其它
英文摘要	Major histocompatibility complex class I (MHC I) molecules play a pivotal role in the immune recognition to intracellular pathogens. A number of important splice variants have already been characterized for these molecules in different species, suggesting their important roles in modulation of immune responses. In this study, we have identified and characterized a novel alternatively spliced form of rhesus macaque MHC IA (designated MHC IA-sv2) that lacks exons coding for the alpha 2 and alpha 3 domains. Despite lacking the alpha 2 and alpha 3 domains, MHC IA-sv2 is targeted to the cell surface, as a 23-kDa glycoprotein that is totally susceptible to endoglycosidase-H digestion and is reduced to 18 kDa after deglycosylation with PNGase F. In contrast, the full-length MHC IA reaches the cell surface as a 43-kDa protein of form with complex-type N-glycosylation (endoglycosidase-H resistant). Moreover, we provide evidence here that MHC IA-sv2 can self-associate, forming homodimers, or associate with the fully mature MHC IA molecule, forming a heterodimeric structure in mammalian cells. These data demonstrate that the formation of heterodimers may have some functional implications in the fine tuning of MHC IA-mediated innate and adaptive immune responses
收录类别	SCI
资助信息	The work was supported by the National Basic Research Program of China (2012CBA01305), the National Natural Science Founda- tion of China (30800113; 30872317; U0832601; 81172876), the Knowledge Innovation Program of CAS (KSCX2-EW-R-13), the Key Scientific and Technological Program of China (2012ZX10001-006; 2012ZX10001-007).
语种	英语
WOS记录号	WOS:000310822500005
公开日期	2013-04-09
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7318]
专题	昆明动物研究所_分子免疫药理学昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of the Chinese Academy of Sciences & Yunnan Province, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, PR China 2.Graduate School of Chinese Academy of Sciences, Beijing 100039, PR China
推荐引用方式 GB/T 7714	Dai ZX,Zhang GH,Zhang XH,et al. Identification and characterization of a novel splice variant of rhesus macaque MHC IA[J]. MOLECULAR IMMUNOLOGY,2013,53(3):206-213.
APA	Dai ZX,Zhang GH,Zhang XH,&Zheng YT[*].(2013).Identification and characterization of a novel splice variant of rhesus macaque MHC IA.MOLECULAR IMMUNOLOGY,53(3),206-213.
MLA	Dai ZX,et al."Identification and characterization of a novel splice variant of rhesus macaque MHC IA".MOLECULAR IMMUNOLOGY 53.3(2013):206-213.