Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos

CORC > 昆明动物研究所 > 昆明动物研究所 > 遗传资源与进化国家重点实验室 > 分子进化基因组学

	Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos
	Chen DY[*]4; Wen DC 4,8; Zhang YP 5; Sun QY 4; Han ZM 4; Liu ZH 4; Shi P 5; Li JS 4; Xiangyu JG 5; Lian L 4
刊名	BIOLOGY OF REPRODUCTION
	2002
卷号	67 期号:2 页码:637-642
关键词	developmental biology early development embryo implantation pregnancy
ISSN号	0006-3363
通讯作者	chendy@panda.ioz.ac.cn
合作状况	其它
英文摘要	Somatic cell nuclei of giant pandas can dedifferentiate in enucleated rabbit ooplasm, and the reconstructed eggs can develop to blastocysts. In order to observe whether these interspecies cloned embryos can implant in the uterus of an animal other than the panda, we transferred approximately 2300 panda-rabbit cloned embryos into 100 synchronized rabbit recipients, and none became pregnant. In another approach, we co-transferred both panda-rabbit and cat-rabbit interspecies cloned embryos into the oviducts of 21 cat recipients. Fourteen recipients exhibited estrus within 35 days; five recipients exhibited estrus 43-48 days after embryo transfer; and the other two recipients died of pneumonia, one of which was found to be pregnant with six early fetuses when an autopsy was performed. Microsatellite DNA analysis of these early fetuses confirmed that two were from giant panda-rabbit cloned embryos. The results demonstrated that panda-rabbit cloned embryos can implant in the uterus of a third species, the domestic cat. By using mitochondrial-specific probes of panda and rabbit, we found that mitochondria from both panda somatic cells and rabbit ooplasm coexisted in early blastocysts, but mitochondria from rabbit ooplasm decreased, and those from panda donor cells dominated in early fetuses after implantation. Our results reveal that mitochondria from donor cells may substitute those from recipient oocytes in postimplanted, interspecies cloned embryos.
收录类别	SCI
资助信息	This research was supported by Climbing special grant 08 of the Climb- ing Project from the Ministry of Science and Technology, China; and by grants KSCX1-05-01 and KSCX2-SW-303 from the Knowledge Innovation Project of the Chinese Academy of Sciences
原文出处	200267637.pdf
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/4049]
专题	昆明动物研究所_分子进化基因组学昆明动物研究所_进化与功能基因组学
作者单位	1.* 2.* 3.** 4.Chinese Acad Sci, Inst Zool, State Key Lab Reprod Biol, Beijing 100080, Peoples R China 5.Chinese Acad Sci, Kunming Inst Zool, Kunming 650223, Peoples R China 6.Fuzhou Giant Panda Res Ctr, Fuzhou 350001, Peoples R China 7.China Res & Conservat Ctr Giant Panada, Sichuan 623006, Peoples R China 8.Guangzhou Med Coll, Guangzhou 510182, Peoples R China
推荐引用方式 GB/T 7714	Chen DY[*],Wen DC,Zhang YP,et al. Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos[J]. BIOLOGY OF REPRODUCTION,2002,67(2):637-642.
APA	Chen DY[*].,Wen DC.,Zhang YP.,Sun QY.,Han ZM.,...&Zhang HM.(2002).Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos.BIOLOGY OF REPRODUCTION,67(2),637-642.
MLA	Chen DY[*],et al."Interspecies implantation and mitochondria fate of panda-rabbit cloned embryos".BIOLOGY OF REPRODUCTION 67.2(2002):637-642.