Dermatophagoides farinae allergens diversity  identification by proteomics

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	Dermatophagoides farinae allergens diversity identification by proteomics
	An S 1,2,5,6; Chen LL 4; Long CB 1,2; Liu XY 3; Xu XM 4; Lu XR 7; Rong MQ[]1,2; Liu ZG[]3; Lai R[*]1,2
刊名	MOLECULAR & CELLULAR PROTEOMICS
	2013
卷号	12 期号:7 页码:1818-1828
关键词	Dermatophagoides farinae Allergen Proteomics Mite
通讯作者	rongmingqiang@mail.kiz.ac.cn ; rlai@mail.kiz.ac.cn ; ,lzg195910@126.com
合作状况	其它
英文摘要	The most important indoor allergens for humans are house dust mites (HDM). Fourteen Dermatophagoides farinae allergens (Der f 1-3, 6, 7, 10, 11, 13-18, and 22) are reported although more than 30 allergens have been estimated in D. farinae. Seventeen allergens belonging to 12 different groups were identified by a procedure of proteomics combined with two-dimensional immunoblotting from D. farina extracts. Their sequences were determined by Edman degradation, mass spectrometry analysis, and cDNA cloning. Their allergenicities were assayed by enzyme-linked immunosorbent assay inhibition tests, immunoblots, basophil activation test, and skin prick tests. Eight of them are the first report as D. farinae allergens. The procedure of using a proteomic approach combined with a purely discovery approach using sera of patients with broad IgE reactivity profiles to mite allergens was an effective method to investigate a more complete repertoire of D. farinae allergens. The identification of eight new D. farinae allergens will be helpful for HDM allergy diagnosis and therapy, especially for patients without response for HDM major allergens. In addition, the current work significantly extendedthe repertoire of D. farinae allergens.
收录类别	SCI
资助信息	This work was supported by Chinese National Natural Science Foundation (31070701, 31000962, 31025025, 31200590, 30971179, 30972848, 31028006), the Ministry of Science and Technology (2010CB529800, 2009ZX09103-1/091, 011ZX09102-002-10, 2013CB911300).
语种	英语
公开日期	2013-09-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7677]
专题	昆明动物研究所_动物毒素室昆明动物研究所_动物模型与人类重大疾病机理重点实验室
作者单位	1.Key Laboratory of Animal Models and Human Disease Mechanisms of Chinese Academy of Science 2.Yunnan Province, Kunming Institute of Zoology, Kunming 650223, Yunnan, China 3.Institute of Allergy and Immunology, Shenzhen University, Shen- zhen, China 4.Clinical Laboratory, the First Affiliated Hospital of Kun- ming Medical College, Kunming 650032, Yunnan, China 5.School of Life Sciences, University of Science and Technology of China, Hefei 230026, Anhui, China 6.Clinical laboratory of Wenshan- zhou People Hospital, Wenshanzhou 663000, Yunnan, China 7.Clinical laboratory of Wenshan- zhou People Hospital, Wenshanzhou 663000, Yunnan, China
推荐引用方式 GB/T 7714	An S,Chen LL,Long CB,et al. Dermatophagoides farinae allergens diversity identification by proteomics[J]. MOLECULAR & CELLULAR PROTEOMICS,2013,12(7):1818-1828.
APA	An S.,Chen LL.,Long CB.,Liu XY.,Xu XM.,...&Lai R[*].(2013).Dermatophagoides farinae allergens diversity identification by proteomics.MOLECULAR & CELLULAR PROTEOMICS,12(7),1818-1828.
MLA	An S,et al."Dermatophagoides farinae allergens diversity identification by proteomics".MOLECULAR & CELLULAR PROTEOMICS 12.7(2013):1818-1828.