Heterorhabditidoides rugaoensis n. sp (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae

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	Heterorhabditidoides rugaoensis n. sp (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae
	Zhang KY[*]1,2; Liu XH 1; Tan JW 1; Wang Y 1; Qiao L 1; Yedid G 1; Dai CS 1; Qiu RL 1; Yan XW 1; Tan HW 1
刊名	JOURNAL OF NEMATOLOGY
	2013
卷号	44 期号:4 页码:348-360
关键词	Entomopathogenic nematode Heterorhabditidoides rugaoensis n. sp Insectivora-group new nematode species pathogenicity phylogeny Oscheius
通讯作者	keyunzhang@njau.edu.cn
合作状况	其它
英文摘要	A novel entomopathogenic nematode species, Heterorhabditidoides rugaoensis n. sp. RG081015, collected from Rugao, China, is described. The new species is morphologically very similar to H. chongmingensis but can be distinguished from it on the basis of some morphological characteristics, combined with molecular data and a cross-hybridization test. Males of the new species can be recognized on the basis of body length averaging 1396.2 mu m; lateral field with one ridge; metastome isoglottoid with one hemispherical swellings comprised of two to three well-developed warts; asymmetric spicules; peloderan bursa. In IJs, EP = 134.5 mu m; ES = 149.3 mu m; tail length = 82.5 mu m; and a = 20.5. Hermaphroditic females have four to five lateral ridges. The 18S rDNA and ITS sequences of the two nematodes share 99% and 98% identity, respectively. Phylogenetic trees of 18S rDNA and ITS indicate that the new species is most closely related to H. chongmingensis; thus, the two nematodes belong to the same genus. Failure of cross-hybridization between them indicates that nematode strain RG081015 is a novel species and is described herein as H. rugaoensis n. sp. The LC50 of the novel species against Galleria mellonella were 24.35 IJs / ml within 48 hours of infection. Morphological characteristics, genetic similarity analyses, and phylogenetic relationships provide strong evidence that some species of Oscheius/Insectivora-group should be reassigned to the genus Heterorhabditidoides.
收录类别	SCI
资助信息	This work was supported by 863 Key projects of Ministry of Science and Technology of People’s Republic of China (2008AA101001) and Jiangsu Nat- ural Science Foundation (BK2006560). We thank Dr. Hongmei Li (Key Labo- ratory of Monitoring and Management of Crop Diseases and Pest Insects; Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China) for positive advice on identification of the new species described in this paper.
WOS记录号	WOS:000320452400006
公开日期	2013-08-08
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7583]
专题	昆明动物研究所_动物毒素室
作者单位	1.Department of Zoology, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China. 2.Key Laboratory of Monitoring and Management of Crop Diseases and Pest Insects, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China 3.Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
推荐引用方式 GB/T 7714	Zhang KY[*],Liu XH,Tan JW,et al. Heterorhabditidoides rugaoensis n. sp (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae[J]. JOURNAL OF NEMATOLOGY,2013,44(4):348-360.
APA	Zhang KY[*].,Liu XH.,Tan JW.,Wang Y.,Qiao L.,...&Gao GF.(2013).Heterorhabditidoides rugaoensis n. sp (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae.JOURNAL OF NEMATOLOGY,44(4),348-360.
MLA	Zhang KY[*],et al."Heterorhabditidoides rugaoensis n. sp (Rhabditida: Rhabditidae), a Novel Highly Pathogenic Entomopathogenic Nematode Member of Rhabditidae".JOURNAL OF NEMATOLOGY 44.4(2013):348-360.