miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing

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	miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing
	Tan T 1,2,3; Zhang YF 1,4; Ji WZ[]1,3; Zheng P[]2
刊名	BIOMED RESEARCH INTERNATIONAL
	2014
卷号	2014 期号:X 页码:e154251
通讯作者	wji@kbimed.com ; zhengp@mail.kiz.ac.cn
合作状况	其它
英文摘要	Spermatogonial stem cells (SSCs) play fundamental roles in spermatogenesis. Although a handful of genes have been discovered as key regulators of SSC self-renewal and differentiation, the regulatory network responsible for SSC function remains unclear. In particular, small RNA signatures during mouse spermatogenesis are not yet systematically investigated. Here, using next generation sequencing, we compared small RNA signatures of in vitro expanded SSCs, testis-derived somatic cells (Sertoli cells), developing germ cells, mouse embryonic stem cells (ESCs), and mouse mesenchymal stem cells among mouse embryonic stem cells (ESCs) to address small RNA transition during mouse spermatogenesis. The results manifest that small RNA transition during mouse spermatogenesis displays overall declined expression profiles of miRNAs and endo-siRNAs, in parallel with elevated expression profiles of piRNAs, resulting in the normal biogenesis of sperms. Meanwhile, several novel miRNAs were preferentially expressed in mouse SSCs, and further investigation of their functional annotation will allow insights into the mechanisms involved in the regulation of SSC activities. We also demonstrated the similarity of miRNA signatures between SSCs and ESCs, thereby providing a new clue to understanding the molecular basis underlying the easy conversion of SSCs to ESCs.
收录类别	SCI
资助信息	This work was supported by the National Program on Key Basic Research Project of China (973 Program; Grant no. 2012CBA01307), the National High-Tech R&D Program of China(863Program;Grantno.2012AA020701),theNational Natural Science Fund for Young Scholars Grant (Grant no. 31101066), and the National Natural Science Fund (Grant no. 31371456).
语种	英语
WOS记录号	WOS:000339764400001
公开日期	2014-09-12
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/7998]
专题	昆明动物研究所_哺乳动物胚胎发育昆明动物研究所_遗传资源与进化国家重点实验室
作者单位	1.Yunnan Key Laboratory of Primate Biomedical Research, No. 1 Boda Road, Yuhua Area, Chenggong District, Kunming, Yunnan 650500, China 2.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan 650223, China 3.Kunming Biomed International and National Engineering Research Center of Biomedicine and Animal Science, Kunming, Yunnan 650500, China 4.Department of Medicine, School of Medicine, Vanderbilt University, Nashville, TN 37203, USA
推荐引用方式 GB/T 7714	Tan T,Zhang YF,Ji WZ[*],et al. miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing[J]. BIOMED RESEARCH INTERNATIONAL,2014,2014(X):e154251.
APA	Tan T,Zhang YF,Ji WZ[],&Zheng P[].(2014).miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing.BIOMED RESEARCH INTERNATIONAL,2014(X),e154251.
MLA	Tan T,et al."miRNA Signature in Mouse Spermatogonial Stem Cells Revealed by High-Throughput Sequencing".BIOMED RESEARCH INTERNATIONAL 2014.X(2014):e154251.