Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory

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	Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory
	Lu ZX 1,2,4; Huang Q 3,4; Su B[*]1,2
刊名	CELL RESEARCH
	2009
卷号	19 期号:2 页码:259-267
关键词	type II KLK8 alternative splicing cognition human evolution
ISSN号	1001-0602
通讯作者	sub@mail.kiz.ac.cn
英文摘要	Kallikrein 8 (KLK8) is a serine protease functioning in the central nervous system, and essential in many aspects of neuronal activities. Sequence comparison and gene expression analysis among diverse primate species identified a human-specific splice form of KLK8 (type II) with preferential expression in the human brain, which may contribute to the origin of human cognition. To gain insights into the physiological and biochemical role of this novel form, we conducted functional analyses of human type II KLK8. Our results show that type II KLK8 is abundantly expressed in human embryonic stem cells and in embryo brain samples, suggesting a potential role in embryogenesis. There are dramatic expression variations in different individuals and brain regions, which is a reflection of its dynamic role in neural activities. Furthermore, the transcription start site (TSS) of KLK8 is tissue-specific, with a brain-specific TSS found in humans indicating functional specialization. Our in vitro biochemical assay shows that there is a type II-specific intermediate protein form, although the processed end-point enzymes are the same for both type I and type II KLK8, suggesting that the emergence of type II KLK8 in the human brain likely leads to functional modifications of KLK8.
学科主题	Cell Biology
收录类别	SCI
资助信息	This study was supported by grants from National Basic Research Program of China (973 Pro- gram) (2006CB701506, 2007CB815705), the Chinese Academy of Sciences (KSCX1-YW-R-34), the National Natural Science Foundation of China (30370755, 30525028 and 30630013), and the Natural Science Foundation of Yunnan Province of China.
语种	英语
公开日期	2010-08-24
内容类型	期刊论文
源URL	[http://159.226.149.42:8088/handle/152453/2197]
专题	昆明动物研究所_比较基因组学昆明动物研究所_遗传资源与进化国家重点实验室昆明动物研究所_中国科学院昆明灵长类研究中心
作者单位	1.State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China 2.Kunming Primate Research Center, Chinese Academy of Sciences, Kunming 650223, China 3.Institute of Bio- chemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China 4.Graduate School of Chinese Academy of Sciences, Beijing 100049, China
推荐引用方式 GB/T 7714	Lu ZX,Huang Q,Su B[*]. Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory[J]. CELL RESEARCH,2009,19(2):259-267.
APA	Lu ZX,Huang Q,&Su B[*].(2009).Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory.CELL RESEARCH,19(2),259-267.
MLA	Lu ZX,et al."Functional characterization of the human-specific (type II) form of kallikrein 8, a gene involved in learning and memory".CELL RESEARCH 19.2(2009):259-267.