Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry

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	Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry
	Qi WS(祁婉舒)1; Guan Q(官青)1; Sun TQ(孙团起)1; CAO YANJING 1; Zhang L(张立)1; Guo YL(郭寅龙)1
刊名	Anal. Chim. Acta
	2015
卷号	870 页码:75-82
其他题名	邻苯二甲酸作为添加剂在提高甲状腺组织中氨基酸的液质检测灵敏度中的应用
通讯作者	孙团起 ; 张立 ; 郭寅龙
英文摘要	In this work, 0.08 mmol L-1 of phthalic acid was introduced as a mobile phase additive to quantify free amino acids (AAs) by hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS). The addition of phthalic acid significantly increased the signal intensity of protonated AA ions, resulting from the decrease of the relative abundance of AA sodium adducts. Meanwhile, the chromatographic peak shapes of AAs were optimized. As a consequence, there was a noticeable increase in the sensitivity of detection for AAs. The limits of detection (LOD) and quantification (LOQ) of the AAs ranged from 0.0500 to 20.0 ng mL (-1) and from 0.100 to 50.0 ng mL(-1), respectively, which were 4-50 times lower compared to the values measured without the addition of phthalic acid. The enhanced detection and separation of AAs were obtained by merely adding phthalic acid to the mobile phase without changing other conditions. Eventually, this simple method was validated and successfully applied to the analysis of twenty-four kinds of free AAs in human thyroid carcinoma and para-carcinoma tissues, demonstrating a significant increase of most AAs in thyroid carcinoma tissues (p < 0.05). (C) 2015 Elsevier B.V. All rights reserved.
学科主题	分析化学
收录类别	SCI
原文出处	http://dx.doi.org/10.1016/j.aca.2015.02.048
语种	英语
WOS记录号	WOS:000351778600007
内容类型	期刊论文
源URL	[http://ir.sioc.ac.cn/handle/331003/39688]
专题	上海有机化学研究所_分析化学研究室
作者单位	1.中科院上海有机化学研究所, 上海质谱中心 2.复旦大学, 上海癌症中心 3.复旦大学, 上海医科大学
推荐引用方式 GB/T 7714	Qi WS,Guan Q,Sun TQ,et al. Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry[J]. Anal. Chim. Acta,2015,870:75-82.
APA	祁婉舒,官青,孙团起,CAO YANJING,张立,&郭寅龙.(2015).Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry.Anal. Chim. Acta,870,75-82.
MLA	祁婉舒,et al."Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry".Anal. Chim. Acta 870(2015):75-82.