Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain

	Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain
	Zhang, Yangyang 1; Wang, Jun 2,3; Liu, Jian'an 1; Han, Juanjuan 1; Xiong, Shaoxiang 1; Yong, Weidong 2,3; Zhao, Zhenwen 1,4
刊名	SCIENTIFIC REPORTS
	2016-05-04
卷号	6
英文摘要	Gangliosides are a family of complex lipids that are abundant in the brain. There is no doubt the investigations about the distribution of gangliosides in brian and the relationship between gangliosides and Alzheimer's disease is profound. However, these investigations are full of challenges due to the structural complexity of gangliosides. In this work, the method for efficient extraction and enrichment of gangliosides from brain was established. Moreover, the distribution of gangliosides in brain was obtained by matrix-assisted laser desorption ionization (MALDI) mass spectrometry imaging (MSI). It was found that 3-aminoquinoline (3-AQ) as matrix was well-suited for MALDI MS analysis of gangliosides in negative ion mode. In addition, the pretreatment by ethanol (EtOH) cleaning brain section and the addition of ammonium formate greatly improved the MS signal of gangliosides in the brain section when MALDI MSI analysis was employed. The distribution of ganliosides in cerebral cortex, hippocampus and cerebellum was respectively acquired by electrospray ionization (ESI) MS and MALDI MSI, and the data were compared for reliability evaluation of MALDI MSI. Further, applying MALDI MSI technology, the distribution of gangliosides in amyloid precursor protein transgenic mouse brain was obtained, which may provide a new insight for bioresearch of Alzheimer's disease (AD).
收录类别	SCI
语种	英语
内容类型	期刊论文
源URL	[http://ir.iccas.ac.cn/handle/121111/36042]
专题	化学研究所_分析测试中心
作者单位	1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, Key Lab Analyt Chem Living Biosyst, Inst Chem,Beijing Mass Spectrum Ctr, Beijing, Peoples R China 2.Chinese Acad Med Sci, Inst Lab Anim Sci, Beijing 100730, Peoples R China 3.Peking Union Med Coll, Beijing 100021, Peoples R China 4.Univ Chinese Acad Sci, Grad Sch, Beijing, Peoples R China
推荐引用方式 GB/T 7714	Zhang, Yangyang,Wang, Jun,Liu, Jian'an,et al. Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain[J]. SCIENTIFIC REPORTS,2016,6.
APA	Zhang, Yangyang.,Wang, Jun.,Liu, Jian'an.,Han, Juanjuan.,Xiong, Shaoxiang.,...&Zhao, Zhenwen.(2016).Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain.SCIENTIFIC REPORTS,6.
MLA	Zhang, Yangyang,et al."Combination of ESI and MALDI mass spectrometry for qualitative, semi-quantitative and in situ analysis of gangliosides in brain".SCIENTIFIC REPORTS 6(2016).