Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage

	Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage
	Y. Fang; R. Z. Zhong; L. Chen; C. T. Feng; H. X. Sun; D. W. Zhou
刊名	Small Ruminant Research
	2015
卷号	130 页码:178-182
通讯作者	周道玮
中文摘要	The aim of this study was to evaluate the effects of astaxanthin (AST) supplementation of semen extender on the quality of ram semen during storage at 4 degrees C for periods up to 72 h. Semen samples were obtained from five Small-tail Han rams by use of artificial vagina during normal reproduction season (August to October in 2014), and diluted with glucose-egg yolk buffer supplemented with 0 (control), 0.5, 1, 2, or 4 mu M AST. The results showed that supplementation of the extender with 2 mu M and 4 mu M of AST increased sperm vitality and plasma membrane integrity (P<0.05), and significantly reduced malondialdehyde and reactive oxygen species levels during the 72 h storage period (P < 0.05). Acrosome integrity did not differ among treatments. After 72 h of storage in extender, samples were analyzed with computer-assisted semen analysis. The motility of sperm in the 2 mu M and 4 mu M AST treatment groups increased significantly (P < 0.05) compared to the control treatment, while curvilinear velocity, progressive velocity, and path velocity were not affected. In conclusion, the present study demonstrated that supplementing semen extender with AST can improve ram semen preservation via protecting plasma membrane integrity. (C) 2015 Published by Elsevier B.V.
内容类型	期刊论文
源URL	[http://159.226.123.10/handle/131322/6465]
专题	东北地理与农业生态研究所_草地农牧业学科组_期刊论文
推荐引用方式 GB/T 7714	Y. Fang,R. Z. Zhong,L. Chen,et al. Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage[J]. Small Ruminant Research,2015,130:178-182.
APA	Y. Fang,R. Z. Zhong,L. Chen,C. T. Feng,H. X. Sun,&D. W. Zhou.(2015).Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage.Small Ruminant Research,130,178-182.
MLA	Y. Fang,et al."Effects of astaxanthin supplementation on the sperm quality and antioxidant capacity of ram semen during liquid storage".Small Ruminant Research 130(2015):178-182.